› 7yiv

X-ray diffraction
3.18Å resolution

The Crystal Structure of Human Tissue Nonspecific Alkaline Phosphatase (ALPL) at Basic pH

Released:
DOI: 10.2210/pdb7yiv/pdb
PDB entry 7yiv coloured by chain, front view.
PDB entry 7yiv coloured by chain, side view.
PDB entry 7yiv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structural pathology for hypophosphatasia caused by malfunctional tissue non-specific alkaline phosphatase.
Yu Y, Rong K, Yao D, Zhang Q, Cao X, Rao B, Xia Y, Lu Y, Shen Y, Yao Y, Xu H, Ma P, Cao Y, Qin A
OpenAccess logo Nat Commun 14 4048 (2023)
PMID: 37422472

Function and Biology Details

Reactions catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
N-phosphocreatine + H(2)O = creatine + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-221680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alkaline phosphatase, tissue-nonspecific isozyme Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 518 amino acids
Theoretical weight: 57.02 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P05186 (Residues: 18-500; Coverage: 95%)
  • Best match: P05186-3 (Residues: 1-445)
Gene name: ALPL
Sequence domains: Alkaline phosphatase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 16 x NAG
Ligand MG 8 x MG
Ligand ZN 16 x ZN
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 158.622Å b: 167.348Å c: 188.783Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.234
Expression system: Trichoplusia ni

Quick links

Citations

1 citations in other articles

Systemic effects of hypophosphatasia characterization of two novel variants in the ALPL gene.
Martínez-Heredia et al. (2023)