› 7z1i

X-ray diffraction
3.09Å resolution

Plant myrosinase TGG1 from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb7z1i/pdb
PDB entry 7z1i coloured by chain, front view.
PDB entry 7z1i coloured by chain, side view.
PDB entry 7z1i coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Gao Y, Farmer E, Jimenez-Sandoval P, Santiago J

Function and Biology Details

Reactions catalysed: 
A thioglucoside + H(2)O = a sugar + a thiol
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-543866 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Myrosinase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 536 amino acids
Theoretical weight: 60.67 KDa
Source organism: Arabidopsis thaliana
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P37702 (Residues: 19-541; Coverage: 100%)
Gene names: At5g26000, BGLU38, T1N24.7, TGG1
Sequence domains: Glycosyl hydrolase family 1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 25 x NAG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 70.989Å b: 88.989Å c: 120.982Å
α: 108.79° β: 92.87° γ: 102.97°
R-values:
R R work R free
0.318 0.317 0.341
Expression system: Trichoplusia ni

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