› 7z8f

Electron Microscopy
20Å resolution

Composite structure of dynein-dynactin-BICDR on microtubules

Released:
DOI: 10.2210/pdb7z8f/pdb
PDB entry 7z8f coloured by chain, front view.
PDB entry 7z8f coloured by chain, side view.
PDB entry 7z8f coloured by chain, top view.
Source organisms:
Primary publication:
Structure of dynein-dynactin on microtubules shows tandem adaptor binding.
Chaaban S, Carter AP
Nature (2022)
PMID: 36071160
Related structures: EMD-14549

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 43-mer (preferred)
PDBe Complex ID:
PDB-CPX-236682 (preferred)
Entry contents:
16 distinct polypeptide molecules
Macromolecules (16 distinct):
Alpha-centractin Chains: A, B, C, D, E, F, G, I
Molecule details ›
Chains: A, B, C, D, E, F, G, I
Length: 376 amino acids
Theoretical weight: 42.67 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F2Z5G5 (Residues: 1-310; Coverage: 89%)
Gene name: ACTR1A
Sequence domains: Actin
Actin, cytoplasmic 1 Chain: H
Molecule details ›
Chain: H
Length: 375 amino acids
Theoretical weight: 41.78 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q6QAQ1 (Residues: 1-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Actin-related protein 10 Chain: J
Molecule details ›
Chain: J
Length: 417 amino acids
Theoretical weight: 46.25 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LHK5 (Residues: 1-417; Coverage: 100%)
Gene names: ACTR10, ARP11
Sequence domains: Actin
F-actin-capping protein subunit alpha-1 Chain: K
Molecule details ›
Chain: K
Length: 286 amino acids
Theoretical weight: 33.06 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0PFK5 (Residues: 1-286; Coverage: 100%)
Gene names: CAPZA1, CP alpha1, Capza
Sequence domains: F-actin capping protein alpha subunit
F-actin-capping protein subunit beta Chain: L
Molecule details ›
Chain: L
Length: 272 amino acids
Theoretical weight: 30.67 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0PFK7 (Residues: 1-272; Coverage: 100%)
Gene name: CAPZB
Sequence domains: F-actin capping protein, beta subunit
Dynactin subunit 2 Chains: M, N, P, Q
Molecule details ›
Chains: M, N, P, Q
Length: 405 amino acids
Theoretical weight: 44.7 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A5G2QD80 (Residues: 1-405; Coverage: 100%)
Gene name: DCTN2
Sequence domains: Dynamitin
Dynactin subunit 3 Chains: O, R
Molecule details ›
Chains: O, R
Length: 186 amino acids
Theoretical weight: 21.19 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SEC0 (Residues: 1-186; Coverage: 100%)
Gene name: DCTN3
Sequence domains: Dynactin subunit p22
Dynactin subunit 1 Chains: S, T
Molecule details ›
Chains: S, T
Length: 1281 amino acids
Theoretical weight: 142.02 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287B8J2 (Residues: 1-1281; Coverage: 100%)
Gene name: DCTN1
Sequence domains:
Dynactin subunit 6 Chain: U
Molecule details ›
Chain: U
Length: 190 amino acids
Theoretical weight: 20.7 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: D0G6S1 (Residues: 1-190; Coverage: 100%)
Gene name: DCTN6
Dynactin subunit 5 Chain: V
Molecule details ›
Chain: V
Length: 182 amino acids
Theoretical weight: 20.15 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A286ZK88 (Residues: 1-182; Coverage: 100%)
Gene name: DCTN5
Sequence domains: Dynactin subunit 5
BICD family-like cargo adapter 1 Chains: W, X, w, x
Molecule details ›
Chains: W, X, w, x
Length: 577 amino acids
Theoretical weight: 65.38 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0JNT9 (Residues: 1-577; Coverage: 100%)
Gene names: Bicdl1, Bicdr1, Ccdc64
Dynactin subunit 4 Chain: Y
Molecule details ›
Chain: Y
Length: 467 amino acids
Theoretical weight: 52.92 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1TB62 (Residues: 1-467; Coverage: 100%)
Gene name: DCTN4
Sequence domains: Dynactin p62 family
Cytoplasmic dynein 1 heavy chain 1 Chains: e, f, m, n
Molecule details ›
Chains: e, f, m, n
Length: 4646 amino acids
Theoretical weight: 533.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q14204 (Residues: 1-4646; Coverage: 100%)
Gene names: DHC1, DNCH1, DNCL, DNECL, DYHC, DYNC1H1, KIAA0325
Sequence domains:
Cytoplasmic dynein 1 intermediate chain 2 Chains: g, h, o, p
Molecule details ›
Chains: g, h, o, p
Length: 638 amino acids
Theoretical weight: 71.55 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13409 (Residues: 1-638; Coverage: 100%)
Gene names: DNCI2, DNCIC2, DYNC1I2
Sequence domains:
Cytoplasmic dynein 1 light intermediate chain 2 Chains: i, j, q, r
Molecule details ›
Chains: i, j, q, r
Length: 492 amino acids
Theoretical weight: 54.17 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43237 (Residues: 1-492; Coverage: 100%)
Gene names: DNCLI2, DYNC1LI2, LIC2
Sequence domains: Dynein light intermediate chain (DLIC)
Dynein light chain roadblock-type 1 Chains: k, l, s, t
Molecule details ›
Chains: k, l, s, t
Length: 96 amino acids
Theoretical weight: 10.93 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NP97 (Residues: 1-96; Coverage: 100%)
Gene names: BITH, DNCL2A, DNLC2A, DYNLRB1, HSPC162, ROBLD1
Sequence domains: Roadblock/LC7 domain

Ligands and Environments

5 bound ligands:
Ligand ADP 13 x ADP
Ligand MG 14 x MG
Ligand ATP 5 x ATP
Ligand ZN 3 x ZN
Ligand ANP 8 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 20Å
Relevant EMDB volumes: EMD-14549
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Microtubule acetylation dyshomeostasis in Parkinson's disease.
Naren et al. (2023)
3 more

5 mentions without citation

Structure and Function of Dynein's Non-Catalytic Subunits.
Rao et al. (2024)
4 more