› 7a4p

Electron Microscopy
4.2Å resolution

Structure of small high-light grown Chlorella ohadii photosystem I

Released:
DOI: 10.2210/pdb7a4p/pdb
PDB entry 7a4p coloured by chain, front view.
PDB entry 7a4p coloured by chain, side view.
PDB entry 7a4p coloured by chain, top view.
Source organism: Chlorella ohadii
Primary publication:
Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii.
Caspy I, Neumann E, Fadeeva M, Liveanu V, Savitsky A, Frank A, Kalisman YL, Shkolnisky Y, Murik O, Treves H, Hartmann V, Nowaczyk MM, Schuhmann W, Rögner M, Willner I, Kaplan A, Schuster G, Nelson N, Lubitz W, Nechushtai R
Nat Plants 7 1314-1322 (2021)
PMID: 34462576
Related structures: EMD-11640

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-496853 (preferred)
Entry contents:
20 distinct polypeptide molecules
Macromolecules (20 distinct):
Photosystem I P700 chlorophyll a apoprotein A1 Chain: A
Molecule details ›
Chain: A
Length: 741 amino acids
Theoretical weight: 82.13 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SY74 (Residues: 11-751; Coverage: 99%)
Gene names: csorokcp_00027, psaA
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I P700 chlorophyll a apoprotein A2 Chain: B
Molecule details ›
Chain: B
Length: 731 amino acids
Theoretical weight: 81.54 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SUA3 (Residues: 3-733; Coverage: 100%)
Gene names: csorokcp_00028, psaB
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I iron-sulfur center Chain: C
Molecule details ›
Chain: C
Length: 80 amino acids
Theoretical weight: 8.72 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SKM2 (Residues: 2-81; Coverage: 99%)
Gene names: csorokcp_00038, psaC
Sequence domains: 4Fe-4S dicluster domain
Photosystem I reaction center subunit chloroplastic Chain: D
Molecule details ›
Chain: D
Length: 143 amino acids
Theoretical weight: 15.96 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TKF8 (Residues: 188-330; Coverage: 43%)
Gene name: C2E21_6785
Sequence domains: PsaD
Photosystem I reaction center subunit IV Chain: E
Molecule details ›
Chain: E
Length: 64 amino acids
Theoretical weight: 7.19 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6U4S6 (Residues: 35-95; Coverage: 61%)
Gene name: C2E21_0167
Sequence domains: Photosystem I reaction centre subunit IV / PsaE
Photosystem I reaction center subunit III Chain: F
Molecule details ›
Chain: F
Length: 165 amino acids
Theoretical weight: 18.07 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TPV8 (Residues: 318-482; Coverage: 34%)
Gene name: C2E21_5099
Sequence domains: Photosystem I reaction centre subunit III
PSI-G Chain: G
Molecule details ›
Chain: G
Length: 99 amino acids
Theoretical weight: 10.33 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TZI8 (Residues: 1228-1326; Coverage: 8%)
Gene name: C2E21_1494
Sequence domains: Photosystem I psaG / psaK
Photosystem I reaction center subunit IX Chain: J
Molecule details ›
Chain: J
Length: 41 amino acids
Theoretical weight: 4.48 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SUD1 (Residues: 1-41; Coverage: 100%)
Gene names: csorokcp_00066, psaJ
Sequence domains: Photosystem I reaction centre subunit IX / PsaJ
PSI-K Chain: K
Molecule details ›
Chain: K
Length: 86 amino acids
Theoretical weight: 8.73 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6U0J1 (Residues: 32-117; Coverage: 74%)
Gene name: C2E21_1903
Sequence domains: Photosystem I psaG / psaK
Photosystem I reaction center subunit XII Chain: M
Molecule details ›
Chain: M
Length: 31 amino acids
Theoretical weight: 3.38 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SU98 (Residues: 1-31; Coverage: 100%)
Gene names: csorokcp_00017, psaM
Sequence domains: Photosystem I protein M (PsaM)
Photosystem I reaction center subunit VIII Chain: I
Molecule details ›
Chain: I
Length: 35 amino acids
Theoretical weight: 3.86 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: W8SIP9 (Residues: 1-35; Coverage: 97%)
Gene names: csorokcp_00005, psaI
Sequence domains: Photosystem I reaction centre subunit VIII
PSI subunit V Chain: L
Molecule details ›
Chain: L
Length: 157 amino acids
Theoretical weight: 16.5 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TC44 (Residues: 295-452; Coverage: 35%)
Gene name: C2E21_9213
Sequence domains: Photosystem I reaction centre subunit XI
Chlorophyll a-b binding protein, chloroplastic Chains: 1, a
Molecule details ›
Chains: 1, a
Length: 192 amino acids
Theoretical weight: 19.93 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TT36 (Residues: 32-223; Coverage: 86%)
Gene name: C2E21_4122
Sequence domains: Chlorophyll A-B binding protein
Glutathione reductase Chain: 3
Molecule details ›
Chain: 3
Length: 241 amino acids
Theoretical weight: 26.16 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TMT4 (Residues: 247-487; Coverage: 12%)
Gene name: C2E21_6000
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 4
Molecule details ›
Chain: 4
Length: 207 amino acids
Theoretical weight: 23.01 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TQ14 (Residues: 30-237; Coverage: 84%)
Gene name: C2E21_5185
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 5
Molecule details ›
Chain: 5
Length: 227 amino acids
Theoretical weight: 25.11 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6U4K1 (Residues: 30-256; Coverage: 88%)
Gene name: C2E21_0580
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 6
Molecule details ›
Chain: 6
Length: 231 amino acids
Theoretical weight: 25.32 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TPR7 (Residues: 35-265; Coverage: 87%)
Gene name: C2E21_5184
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 7
Molecule details ›
Chain: 7
Length: 221 amino acids
Theoretical weight: 24.02 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TS63 (Residues: 6-226; Coverage: 81%)
Gene name: C2E21_4121
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 8
Molecule details ›
Chain: 8
Length: 219 amino acids
Theoretical weight: 23.61 KDa
Source organism: Chlorella ohadii
UniProt:
  • Canonical: A0A2P6TZ50 (Residues: 29-247; Coverage: 88%)
Gene name: C2E21_2201
Sequence domains: Chlorophyll A-B binding protein
Photosystem I reaction center subunit VI-chloroplastic-like Chain: H
Molecule details ›
Chain: H
Length: 3 amino acids
Theoretical weight: 329 Da
Source organism: Chlorella ohadii

Ligands and Environments

29 bound ligands:
Ligand CL0 1 x CL0
Ligand CLA 200 x CLA
Ligand PQN 2 x PQN
Ligand BCR 31 x BCR
Ligand LHG 20 x LHG
Ligand DGD 3 x DGD
Ligand 3PH 1 x 3PH
Ligand SF4 3 x SF4
Ligand LMT 3 x LMT
Ligand PCW 2 x PCW
Ligand PTY 5 x PTY
Ligand LAP 3 x LAP
Ligand SQD 2 x SQD
Ligand ERG 1 x ERG
Ligand RRX 1 x RRX
Ligand LPX 2 x LPX
Ligand ECH 1 x ECH
Ligand LUT 18 x LUT
Ligand CHL 22 x CHL
Ligand OLA 2 x OLA
Ligand QTB 2 x QTB
Ligand GG0 1 x GG0
Ligand PLM 2 x PLM
Ligand DGA 2 x DGA
Ligand SPH 1 x SPH
Ligand XAT 1 x XAT
Ligand 4RF 3 x 4RF
Ligand C7Z 1 x C7Z
Ligand P5S 1 x P5S
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-11640

Quick links

Citations

3 review citations

LHC-like Proteins: The Guardians of Photosynthesis.
Levin et al. (2023)
2 more

1 mention without citation

Investigating the Balance between Structural Conservation and Functional Flexibility in Photosystem I.
Nelson N. (2024)