› 7a6s

X-ray diffraction
1.75Å resolution

Crystal Structure of Asn173Ser variant of Human Deoxyhypusine Synthase

Released:
DOI: 10.2210/pdb7a6s/pdb
PDB entry 7a6s coloured by chain, front view.
PDB entry 7a6s coloured by chain, side view.
PDB entry 7a6s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of human eIF5A-DHS complex reveals the molecular basis of hypusination-associated neurodegenerative disorders.
Wątor E, Wilk P, Biela A, Rawski M, Zak KM, Steinchen W, Bange G, Glatt S, Grudnik P
OpenAccess logo Nat Commun 14 1698 (2023)
PMID: 36973244

Function and Biology Details

Reaction catalysed: 
(1a) spermidine + NAD(+) = dehydrospermidine + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyhypusine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 369 amino acids
Theoretical weight: 41.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P49366 (Residues: 1-369; Coverage: 100%)
Gene names: DHPS, DS
Sequence domains: Deoxyhypusine synthase

Ligands and Environments

3 bound ligands:
Ligand EDO 7 x EDO
Ligand ACT 2 x ACT
Ligand OXM 1 x OXM
1 modified residue:
Ligand CSS 2 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3221
Unit cell:
a: 105.382Å b: 105.382Å c: 159.902Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.162 0.199
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

A global view of the human post-translational modification landscape.
Kitamura et al. (2023)