› 7a7a

Electron Microscopy
3.08Å resolution

Cryo-EM structure of W107R after heme uptake (2heme molecules) KatG from M. tuberculosis

Released:
DOI: 10.2210/pdb7a7a/pdb
PDB entry 7a7a coloured by chain, front view.
PDB entry 7a7a coloured by chain, side view.
PDB entry 7a7a coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Using cryo-EM to understand antimycobacterial resistance in the catalase-peroxidase (KatG) from Mycobacterium tuberculosis.
Munir A, Wilson MT, Hardwick SW, Chirgadze DY, Worrall JAR, Blundell TL, Chaplin AK
OpenAccess logo Structure 29 899-912.e4 (2021)
PMID: 33444527
Related structures: EMD-11676

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase-peroxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 740 amino acids
Theoretical weight: 80.66 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIE5 (Residues: 1-740; Coverage: 100%)
Gene names: MTCY180.10, Rv1908c, katG
Sequence domains: Peroxidase

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.08Å
Relevant EMDB volumes: EMD-11676
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Recent Insights into the Structure and Function of Mycobacterial Membrane Proteins Facilitated by Cryo-EM.
Bendre et al. (2021)
2 more

1 mention without citation

Using cryo-EM to understand antimycobacterial resistance in the catalase-peroxidase (KatG) from Mycobacterium tuberculosis.
Munir et al. (2021)