› 7a9j

X-ray diffraction
1.72Å resolution

Crystal structure of the R395G mutant form of Coronafacic Acid Ligase from Pectobacterium brasiliense

Released:
DOI: 10.2210/pdb7a9j/pdb
PDB entry 7a9j coloured by chain, front view.
PDB entry 7a9j coloured by chain, side view.
PDB entry 7a9j coloured by chain, top view.
Source organism: Pectobacterium brasiliense
Primary publication:
Discovery, characterization and engineering of ligases for amide synthesis.
Winn M, Rowlinson M, Wang F, Bering L, Francis D, Levy C, Micklefield J
Nature 593 391-398 (2021)
PMID: 34012085

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ligase Chain: A
Molecule details ›
Chain: A
Length: 537 amino acids
Theoretical weight: 60.31 KDa
Source organism: Pectobacterium brasiliense
Expression system: Escherichia coli
UniProt:
  • Canonical: M4GWN4 (Residues: 1-516; Coverage: 100%)
Gene names: KCO_08370, KU74_07105, cfl
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand R4Z 1 x R4Z
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 77.611Å b: 115.167Å c: 71.013Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.208
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Transformative Power of Biocatalysis in Convergent Synthesis.
Zetzsche et al. (2022)
1 more

1 mention without citation

All DACs in a Row: Domain Architectures of Bacterial and Archaeal Diadenylate Cyclases.
Galperin MY. (2023)