› 7abr

Electron Microscopy
3.7Å resolution

Cryo-EM structure of B. subtilis ClpC (DWB mutant) hexamer bound to a substrate polypeptide

Released:
DOI: 10.2210/pdb7abr/pdb
PDB entry 7abr coloured by chain, front view.
PDB entry 7abr coloured by chain, side view.
PDB entry 7abr coloured by chain, top view.
Source organisms:
Primary publication:
BacPROTACs mediate targeted protein degradation in bacteria.
Morreale FE, Kleine S, Leodolter J, Junker S, Hoi DM, Ovchinnikov S, Okun A, Kley J, Kurzbauer R, Junk L, Guha S, Podlesainski D, Kazmaier U, Boehmelt G, Weinstabl H, Rumpel K, Schmiedel VM, Hartl M, Haselbach D, Meinhart A, Kaiser M, Clausen T
OpenAccess logo Cell 185 2338-2353.e18 (2022)
PMID: 35662409
Related structures: EMD-11707

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153437 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Negative regulator of genetic competence ClpC/MecB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 818 amino acids
Theoretical weight: 91.21 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P37571 (Residues: 1-810; Coverage: 100%)
Gene names: BSU00860, clpC, mecB
Sequence domains:
substrate polypeptide Chain: S
Molecule details ›
Chain: S
Length: 26 amino acids
Theoretical weight: 2.23 KDa
Source organism: unidentified
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ADP 4 x ADP
Ligand ATP 7 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-11707
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Ligandability of E3 Ligases for Targeted Protein Degradation Applications.
Belcher et al. (2023)
11 more

2 mentions without citation

BacPROTACs mediate targeted protein degradation in bacteria.
Morreale et al. (2022)
1 more

Experimental raw data

Links to raw experimental data available for this entry are listed below

Electron microscopy data related to PDB entry 7abr found at the Electron Microscopy Public Image Archive (EMPIAR)

Data DOI: 10.6019/EMPIAR-11068
Datasets: 1
Total size: 4900Gb
EMPIAR thumbnail