› 7ado

Electron Microscopy
3.39Å resolution

Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs

Released:
DOI: 10.2210/pdb7ado/pdb
PDB entry 7ado coloured by chain, front view.
PDB entry 7ado coloured by chain, side view.
PDB entry 7ado coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.
Miller-Vedam LE, Bräuning B, Popova KD, Schirle Oakdale NT, Bonnar JL, Prabu JR, Boydston EA, Sevillano N, Shurtleff MJ, Stroud RM, Craik CS, Schulman BA, Frost A, Weissman JS
OpenAccess logo Elife 9 (2020)
PMID: 33236988
Related structures: EMD-11732

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279351 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
ER membrane protein complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 993 amino acids
Theoretical weight: 111.89 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N766 (Residues: 1-993; Coverage: 100%)
Gene names: EMC1, KIAA0090, PSEC0263
Sequence domains:
ER membrane protein complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 297 amino acids
Theoretical weight: 34.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15006 (Residues: 1-297; Coverage: 100%)
Gene names: EMC2, KIAA0103, TTC35
Sequence domains: EMC2 TPR-like repeat domain
ER membrane protein complex subunit 3 Chain: C
ER membrane protein complex subunit 4 Chain: D
ER membrane protein complex subunit 5 Chain: E
ER membrane protein complex subunit 6 Chain: F
Endoplasmic reticulum membrane protein complex subunit 7 Chain: G
ER membrane protein complex subunit 8 Chain: H
ER membrane protein complex subunit 10 Chain: I
Unassigned helix Chain: K

Ligands and Environments

2 bound ligands:
Ligand NAG 4 x NAG
Ligand PCW 5 x PCW
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.39Å
Relevant EMDB volumes: EMD-11732
Expression system: Homo sapiens

Quick links

Citations

13 review citations

The mechanisms of integral membrane protein biogenesis.
Hegde et al. (2022)
12 more

5 mentions without citation

Cryo-EM structures of the endoplasmic reticulum membrane complex.
Bai et al. (2022)
4 more