7ado

Electron Microscopy
3.39Å resolution

Cryo-EM structure of human ER membrane protein complex in lipid nanodiscs

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-279351 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
ER membrane protein complex subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 993 amino acids
Theoretical weight: 111.89 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N766 (Residues: 1-993; Coverage: 100%)
Gene names: EMC1, KIAA0090, PSEC0263
Sequence domains:
ER membrane protein complex subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 297 amino acids
Theoretical weight: 34.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q15006 (Residues: 1-297; Coverage: 100%)
Gene names: EMC2, KIAA0103, TTC35
Sequence domains: EMC2 TPR-like repeat domain
ER membrane protein complex subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 261 amino acids
Theoretical weight: 29.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9P0I2 (Residues: 1-261; Coverage: 100%)
Gene names: EMC3, TMEM111
Sequence domains: Integral membrane protein EMC3/TMCO1-like
ER membrane protein complex subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 183 amino acids
Theoretical weight: 20.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5J8M3 (Residues: 1-183; Coverage: 100%)
Gene names: EMC4, HSPC184, PIG17, TMEM85
Sequence domains: Protein of unknown function (DUF1077)
ER membrane protein complex subunit 5 Chain: E
Molecule details ›
Chain: E
Length: 139 amino acids
Theoretical weight: 15.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N4V1 (Residues: 1-131; Coverage: 100%)
Gene names: EMC5, MMGT1, TMEM32
Sequence domains: Membrane magnesium transporter
ER membrane protein complex subunit 6 Chain: F
Molecule details ›
Chain: F
Length: 110 amino acids
Theoretical weight: 12.03 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BV81 (Residues: 1-110; Coverage: 100%)
Gene names: EMC6, TMEM93
Sequence domains: EMC6
Endoplasmic reticulum membrane protein complex subunit 7 Chain: G
Molecule details ›
Chain: G
Length: 242 amino acids
Theoretical weight: 26.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NPA0 (Residues: 1-242; Coverage: 100%)
Gene names: C11orf3, C15orf24, EMC7, HT022, UNQ905/PRO1926
Sequence domains: ER membrane protein complex subunit 7, beta-sandwich domain
ER membrane protein complex subunit 8 Chain: H
Molecule details ›
Chain: H
Length: 210 amino acids
Theoretical weight: 23.81 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O43402 (Residues: 1-210; Coverage: 100%)
Gene names: C16orf2, C16orf4, COX4AL, COX4NB, EMC8, FAM158B, NOC4
Sequence domains: Uncharacterised protein family (UPF0172)
ER membrane protein complex subunit 10 Chain: I
Molecule details ›
Chain: I
Length: 263 amino acids
Theoretical weight: 27.45 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5UCC4 (Residues: 2-262; Coverage: 100%)
Gene names: C19orf63, EMC10, INM02, UNQ764/PRO1556
Sequence domains: ER membrane protein complex subunit 10
Unassigned helix Chain: K
Molecule details ›
Chain: K
Length: 16 amino acids
Theoretical weight: 1.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.39Å
Relevant EMDB volumes: EMD-11732
Expression system: Homo sapiens