› 7afv

X-ray diffraction
2.4Å resolution

Crystal structure of tetrameric beta-2-microglobulin deltaN6 S52C stabilized by a covalent ligand

Released:
DOI: 10.2210/pdb7afv/pdb
PDB entry 7afv coloured by chain, front view.
PDB entry 7afv coloured by chain, side view.
PDB entry 7afv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Modulation of Amyloidogenic Protein Self-Assembly Using Tethered Small Molecules.
Cawood EE, Guthertz N, Ebo JS, Karamanos TK, Radford SE, Wilson AJ
OpenAccess logo J Am Chem Soc 142 20845-20854 (2020)
PMID: 33253560

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-2-microglobulin Chains: A, B
Molecule details ›
Chains: A, B
Length: 91 amino acids
Theoretical weight: 10.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 27-116; Coverage: 91%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain

Ligands and Environments

2 bound ligands:
Ligand TRS 1 x TRS
Ligand SJK 2 x SJK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P43212
Unit cell:
a: 87.572Å b: 87.572Å c: 56.458Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Visualizing and trapping transient oligomers in amyloid assembly pathways.
Cawood et al. (2021)
3 more

1 mention without citation

Modulation of Amyloidogenic Protein Self-Assembly Using Tethered Small Molecules.
Cawood et al. (2020)