› 7ag6

X-ray diffraction
2.08Å resolution

Crystal structure of SF kinase YihV from E. coli in complex with sulfofructose (SF), ADP-Mg

Released:
DOI: 10.2210/pdb7ag6/pdb
PDB entry 7ag6 coloured by chain, front view.
PDB entry 7ag6 coloured by chain, side view.
PDB entry 7ag6 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Molecular Basis of Sulfosugar Selectivity in Sulfoglycolysis.
Sharma M, Abayakoon P, Epa R, Jin Y, Lingford JP, Shimada T, Nakano M, Mui JW, Ishihama A, Goddard-Borger ED, Davies GJ, Williams SJ
OpenAccess logo ACS Cent Sci 7 476-487 (2021)
PMID: 33791429

Function and Biology Details

Reaction catalysed: 
ATP + 6-deoxy-6-sulfo-D-fructose = ADP + 6-deoxy-6-sulfo-D-fructose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-542631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfofructose kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 32.83 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32143 (Residues: 1-298; Coverage: 100%)
Gene names: JW5568, b3883, yihV
Sequence domains: pfkB family carbohydrate kinase

Ligands and Environments

3 bound ligands:
Ligand ADP 2 x ADP
Ligand RB8 2 x RB8
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 94.88Å b: 94.88Å c: 167.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Implications of hydrogen sulfide in colorectal cancer: Mechanistic insights and diagnostic and therapeutic strategies.
Lin et al. (2023)
1 more