› 7ahh

Electron Microscopy
3.5Å resolution

OpuA inhibited inward-facing, SBD docked

Released:
DOI: 10.2210/pdb7ahh/pdb
PDB entry 7ahh coloured by chain, front view.
PDB entry 7ahh coloured by chain, side view.
PDB entry 7ahh coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis
Primary publication:
Gating by ionic strength and safety check by cyclic-di-AMP in the ABC transporter OpuA.
Sikkema HR, van den Noort M, Rheinberger J, de Boer M, Krepel ST, Schuurman-Wolters GK, Paulino C, Poolman B
OpenAccess logo Sci Adv 6 (2020)
PMID: 33208376
Related structures: EMD-11786

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + quaternary amine-[quaternary amine-binding protein](Side 1) = ADP + phosphate + quaternary amine(Side 2) + [quaternary amine-binding protein](Side 1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102781 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ABC transmembrane type-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 585 amino acids
Theoretical weight: 63.44 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Lactococcus lactis subsp. lactis
UniProt:
  • Canonical: A0A0V8ETW8 (Residues: 1-573; Coverage: 100%)
Gene names: LL229_1537, LL275_1486, N42_0725
Sequence domains:
Glycine betaine transport ATP-binding protein OpuAA Chains: C, D
Molecule details ›
Chains: C, D
Length: 408 amino acids
Theoretical weight: 45.78 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Lactococcus lactis subsp. lactis
UniProt:
  • Canonical: Q9KIF7 (Residues: 1-1, 3-408; Coverage: 100%)
Gene names: L74195, LL1452, busAA, opuAA
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand BET 1 x BET
Ligand ANP 2 x ANP
Ligand 2BA 1 x 2BA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-11786
Expression system: Lactococcus lactis subsp. lactis

Quick links

Citations

4 review citations

Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective.
Bailoni et al. (2023)
3 more

2 mentions without citation

Lactococcus lactis, an Attractive Cell Factory for the Expression of Functional Membrane Proteins.
Frelet-Barrand A. (2022)
1 more