7am2

Electron Microscopy
3.4Å resolution

Intermediate assembly of the Large subunit from Leishmania major mitochondrial ribosome

Released:
Source organism: Leishmania tarentolae
Related structures: EMD-11821

Function and Biology Details

Reactions catalysed:
UTP + RNA(n) = diphosphate + RNA(n+1)
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 78-mer (preferred)
PDBe Complex ID:
PDB-CPX-275719 (preferred)
Entry contents:
73 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (77 distinct):
Large ribosomal subunit protein uL3m Chain: A
Molecule details ›
Chain: A
Length: 467 amino acids
Theoretical weight: 54.03 KDa
Source organism: Leishmania tarentolae
Large ribosomal subunit protein uL4m Chain: B
Molecule details ›
Chain: B
Length: 436 amino acids
Theoretical weight: 50 KDa
Source organism: Leishmania tarentolae
Ribosomal protein L9 domain-containing protein Chain: C
Putative ribosomal protein L11 Chain: E
50S ribosomal protein L13-like protein Chain: F
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chain: G
Putative 50S ribosomal protein L17 Chain: I
bL19m Chain: J
bL20m Chain: K
Large ribosomal subunit protein bL21m Chain: L
Ribosomal protein L22p/L17e Chain: M
Large ribosomal subunit protein uL23m Chain: N
KOW domain-containing protein Chain: O
39S ribosomal protein L28, mitochondrial Chain: Q
Large ribosomal subunit protein uL29m Chain: R
Ribosomal protein L30 ferredoxin-like fold domain-containing protein Chain: S
bL32m Chain: T
bL35m Chain: V
mL41 Chain: Z
Rhodanese domain-containing protein Chain: BA
TRUD domain-containing protein Chain: CA
UA Chain: UA
mL95 Chain: BB
Oligomerization domain containing protein Chain: CB
mL67 Chain: BK
mL71 Chain: BQ
mL81 Chain: BN
mL98 Chain: BE
Putative ribosomal protein L14 Chain: BO
mL86 Chain: At
mL87 Chain: Au
Ribosomal protein L9 domain-containing protein Chain: Ae
mL63 Chain: Af
mL68 Chain: Ah
mL80 Chain: Ap
mL74 Chain: Al
Large ribosomal subunit protein mL43 Chain: Ab
mL42 Chain: Aa
mL52,mL52 Chain: BP
mL93 Chain: Az
Mitochondrial RNA binding complex 1 subunit Chain: Am
mL85 Chain: As
Flagellar attachment zone protein Chain: BG
mL49 Chain: Ad
mL89 Chain: Aw
Peptidyl-prolyl cis-trans isomerase Chain: BH
mL72 Chain: Aj
mL84 Chain: Ar
mL76 Chain: An
mL99 Chain: BF
mL88 Chain: Av
mL70 Chain: BM
mL59/64 Chain: Ag
Peptidyl-prolyl cis-trans isomerase Chain: Bl
LIM zinc-binding domain-containing protein Chain: Ax
J domain-containing protein Chain: BS
tRNA/rRNA methyltransferase SpoU type domain-containing protein Chains: BX, BY
Pseudouridylate synthase RPUSD4, mitochondrial Chain: BZ
Acyl carrier protein Chain: CC
L0R8F8 Chain: CD
G domain-containing protein Chain: BT
GTPase Der Chain: BU
DEAD/DEAH box helicase-like protein Chain: BW
G domain-containing protein Chain: BV
mL78 Chain: U7
U6 Chain: U6
U1 Chain: U1
U3 Chain: U3
U4 Chain: U4
U5 Chain: U5
mL78 Chain: BR
U2 Chain: U2
U8 Chain: U8
Ribosomal RNA Chain: 1
R1 Chain: R1
R2 Chain: R2
R5 Chain: R5

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-11821