7ap8

Electron Microscopy
3.15Å resolution

Atomic structure of the poxvirus initially transcribing complex in conformation 2

Released:
Source organism: Vaccinia virus GLV-1h68
Primary publication:
Structural basis of the complete poxvirus transcription initiation process.
Nat Struct Mol Biol 28 779-788 (2021)
PMID: 34556871
Related structures: EMD-11850

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-107288 (preferred)
Entry contents:
9 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (12 distinct):
DNA-directed RNA polymerase 147 kDa polypeptide Chain: A
DNA-directed RNA polymerase Chain: B
Molecule details ›
Chain: B
Length: 1164 amino acids
Theoretical weight: 133.53 KDa
Source organism: Vaccinia virus GLV-1h68
UniProt:
  • Canonical: Q49PH2 (Residues: 1-1164; Coverage: 100%)
Gene name: mO183R
Sequence domains:
DNA-directed RNA polymerase 35 kDa subunit Chain: C
DNA-directed RNA polymerase subunit Chain: E
DNA-directed RNA polymerase 19 kDa subunit Chain: F
DNA-directed RNA polymerase 18 kDa subunit Chain: G
RNA polymerase-associated transcription-specificity factor RAP94 Chain: I
DNA-directed RNA polymerase 7 kDa subunit Chain: J
DNA-directed RNA polymerase 30 kDa polypeptide Chain: S
Synthetic promoter DNA oligomer, non-template strand Chain: N
Synthetic promoter DNA oligomer, template strand Chain: T
RNA (5'-R(P*AP*UP*AP*AP*A)-3') Chain: P

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.15Å
Relevant EMDB volumes: EMD-11850