› 7arj

Electron Microscopy
3.2Å resolution

LolCDE in complex with lipoprotein and AMPPNP complex undimerized form

Released:
DOI: 10.2210/pdb7arj/pdb
PDB entry 7arj coloured by chain, front view.
PDB entry 7arj coloured by chain, side view.
PDB entry 7arj coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis for bacterial lipoprotein relocation by the transporter LolCDE.
Tang X, Chang S, Zhang K, Luo Q, Zhang Z, Wang T, Qiao W, Wang C, Shen C, Zhang Z, Zhu X, Wei X, Dong C, Zhang X, Dong H
Nat Struct Mol Biol 28 347-355 (2021)
PMID: 33782615
Related structures: EMD-11884

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142347 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Lipoprotein-releasing system transmembrane protein LolC Chain: C
Molecule details ›
Chain: C
Length: 399 amino acids
Theoretical weight: 43.3 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADC3 (Residues: 1-399; Coverage: 100%)
Gene names: JW5161, b1116, lolC, ycfU
Sequence domains:
Lipoprotein-releasing system transmembrane protein LolE Chain: E
Molecule details ›
Chain: E
Length: 414 amino acids
Theoretical weight: 45.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P75958 (Residues: 1-414; Coverage: 100%)
Gene names: JW1104, b1118, lolE, ycfW
Sequence domains:
Lipoprotein-releasing system ATP-binding protein LolD Chains: D, F
Molecule details ›
Chains: D, F
Length: 241 amino acids
Theoretical weight: 26.58 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P75957 (Residues: 1-233; Coverage: 100%)
Gene names: JW5162, b1117, lolD, ycfV
Sequence domains: ABC transporter
Major outer membrane lipoprotein Lpp Chain: V
Molecule details ›
Chain: V
Length: 10 amino acids
Theoretical weight: 1.08 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69776 (Residues: 21-30; Coverage: 17%)
Gene names: JW1667, b1677, lpp, mlpA, mulI

Ligands and Environments

4 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
Ligand Z41 1 x Z41
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-11884
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

ABC Transporters in Bacterial Nanomachineries.
Bilsing et al. (2023)
2 more