› 7ati

X-ray diffraction
1.51Å resolution

Crystal structure of dimeric chlorite dismutase variant Q74V (CCld Q74V) from Cyanothece sp. PCC7425

Released:
DOI: 10.2210/pdb7ati/pdb
PDB entry 7ati coloured by chain, front view.
PDB entry 7ati coloured by chain, side view.
PDB entry 7ati coloured by chain, top view.
Source organism: Cyanothece sp. PCC 7425
Primary publication:
Arresting the Catalytic Arginine in Chlorite Dismutases: Impact on Heme Coordination, Thermal Stability, and Catalysis.
Schmidt D, Serra I, Mlynek G, Pfanzagl V, Hofbauer S, Furtmüller PG, Djinović-Carugo K, Van Doorslaer S, Obinger C
OpenAccess logo Biochemistry 60 621-634 (2021)
PMID: 33586945

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chlorite dismutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 21.8 KDa
Source organism: Cyanothece sp. PCC 7425
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8HNS6 (Residues: 2-182; Coverage: 100%)
Gene name: Cyan7425_1434
Sequence domains: Chlorite dismutase

Ligands and Environments

3 bound ligands:
Ligand GOL 6 x GOL
Ligand HEM 2 x HEM
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 55.014Å b: 72.676Å c: 60.169Å
α: 90° β: 112.733° γ: 90°
R-values:
R R work R free
0.175 0.175 0.193
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Metalloprotein catalysis: structural and mechanistic insights into oxidoreductases from neutron protein crystallography.
Schröder et al. (2021)
1 other article

2 mentions without citation

Structure-Based Statistical Mechanical Model Accounts for the Causality and Energetics of Allosteric Communication.
Guarnera et al. (2016)
1 more