› 7auc

X-ray diffraction
1.53Å resolution

Crystal structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM)

Released:
DOI: 10.2210/pdb7auc/pdb
PDB entry 7auc coloured by chain, front view.
PDB entry 7auc coloured by chain, side view.
PDB entry 7auc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Uncovering an allosteric mode of action for a selective inhibitor of human Bloom syndrome protein.
Chen X, Ali YI, Fisher CE, Arribas-Bosacoma R, Rajasekaran MB, Williams G, Walker S, Booth JR, Hudson JJ, Roe SM, Pearl LH, Ward SE, Pearl FM, Oliver AW
OpenAccess logo Elife 10 (2021)
PMID: 33647232

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-547272 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RecQ-like DNA helicase BLM Chain: A
Molecule details ›
Chain: A
Length: 562 amino acids
Theoretical weight: 63.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54132 (Residues: 636-1070, 1202-1298; Coverage: 38%)
Gene names: BLM, RECQ2, RECQL3
Sequence domains:

Ligands and Environments

8 bound ligands:
Ligand ACT 4 x ACT
Ligand EDO 6 x EDO
Ligand PG4 2 x PG4
Ligand GOL 1 x GOL
Ligand ADP 1 x ADP
Ligand MG 2 x MG
Ligand ZN 1 x ZN
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 54.28Å b: 107.69Å c: 55.2Å
α: 90° β: 109.31° γ: 90°
R-values:
R R work R free
0.19 0.189 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

AlphaFold, Artificial Intelligence (AI), and Allostery.
Nussinov et al. (2022)
1 more