› 7auw

X-ray diffraction
2.8Å resolution

Inhibitory complex of human meprin beta with mouse fetuin-B.

Released:
DOI: 10.2210/pdb7auw/pdb
PDB entry 7auw coloured by chain, front view.
PDB entry 7auw coloured by chain, side view.
PDB entry 7auw coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of a 250-kDa heterotetrameric particle explains inhibition of sheddase meprin β by endogenous fetuin-B.
Eckhard U, Körschgen H, von Wiegen N, Stöcker W, Gomis-Rüth FX
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33782129

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including azocasein, and peptides. Hydrolysis of -His(5)-|-Leu-, -Leu(6)-|-Cys-, -Ala(14)-|-Leu- and -Cys(19)-|-Gly- bonds in insulin B chain.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172585 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (10 distinct):
Meprin A subunit beta Chains: A, C
Molecule details ›
Chains: A, C
Length: 550 amino acids
Theoretical weight: 62.47 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q16820 (Residues: 62-608; Coverage: 81%)
Gene name: MEP1B
Sequence domains:
Fetuin-B Chains: B, D
Molecule details ›
Chains: B, D
Length: 394 amino acids
Theoretical weight: 43.59 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9QXC1 (Residues: 1-388; Coverage: 100%)
Gene name: Fetub
Sequence domains: Cystatin domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
4 bound ligands:
Ligand NA 2 x NA
Ligand ZN 2 x ZN
Ligand SO4 2 x SO4
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 126.66Å b: 88.24Å c: 156.66Å
α: 90° β: 113.82° γ: 90°
R-values:
R R work R free
0.237 0.236 0.261
Expression system: Trichoplusia ni

Quick links

Citations

5 citation in other articles

Helical ultrastructure of the metalloprotease meprin α in complex with a small molecule inhibitor.
Bayly-Jones et al. (2022)
4 more