› 7awm

X-ray diffraction
3.25Å resolution

Structure of the thermostabilized EAAT1 cryst mutant in complex with L-ASP, three sodium ions and the allosteric inhibitor UCPH101

Released:
DOI: 10.2210/pdb7awm/pdb
PDB entry 7awm coloured by chain, front view.
PDB entry 7awm coloured by chain, side view.
PDB entry 7awm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The ion-coupling mechanism of human excitatory amino acid transporters.
Canul-Tec JC, Kumar A, Dhenin J, Assal R, Legrand P, Rey M, Chamot-Rooke J, Reyes N
EMBO J 41 e108341 (2022)
PMID: 34747040

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Excitatory amino acid transporter 1; Neutral amino acid transporter B(0) Chain: A
Molecule details ›
Chain: A
Length: 522 amino acids
Theoretical weight: 56.51 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P43003 (Residues: 1-45, 47-49, 59-61, 66-72, 76-95, 100-100, 102-104, 106-109, 114-117, 121-136, 139-140, 142-142, 144-148, 250-251, 255-255, 258-258, 260-260, 262-265, 274-277, 283-309, 311-314, 316-328, 331-335, 347-351, 353-385, 387-407, 411-421, 424-456, 459-473, 475-487, 493-508, 510-542; Coverage: 66%)
  • Canonical: Q15758 (Residues: 50-50, 54-62, 66-69, 86-88, 104-107, 109-109, 113-113, 118-121, 126-128, 145-146, 149-149, 151-151, 157-237, 240-242, 244-245, 247-247, 249-249, 254-261, 266-270, 298-298, 303-303, 317-318, 324-334, 340-340, 374-374, 396-398, 410-411, 445-446, 462-462, 476-480, 498-498; Coverage: 31%)
Gene names: ASCT2, EAAT1, GLAST, GLAST1, M7V1, RDR, RDRC, SLC1A3, SLC1A5
Sequence domains: Sodium:dicarboxylate symporter family

Ligands and Environments

4 bound ligands:
Ligand NA 3 x NA
Ligand ASP 1 x ASP
Ligand 6Z6 1 x 6Z6
Ligand BA 1 x BA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P63
Unit cell:
a: 122.882Å b: 122.882Å c: 90.032Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.219 0.238
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Stress-Induced Depression and Alzheimer's Disease: Focus on Astrocytes.
Dolotov et al. (2022)
7 other articles

1 mention without citation

The ion-coupling mechanism of human excitatory amino acid transporters.
Canul-Tec et al. (2022)