› 7azl

X-ray diffraction
2.42Å resolution

DNA polymerase sliding clamp from Escherichia coli with peptide 38 bound

Released:
DOI: 10.2210/pdb7azl/pdb
PDB entry 7azl coloured by chain, front view.
PDB entry 7azl coloured by chain, side view.
PDB entry 7azl coloured by chain, top view.
Source organisms:
Primary publication:
Iterative Structure-Based Optimization of Short Peptides Targeting the Bacterial Sliding Clamp.
Monsarrat C, Compain G, André C, Engilberge S, Martiel I, Oliéric V, Wolff P, Brillet K, Landolfo M, Silva da Veiga C, Wagner J, Guichard G, Burnouf DY
J Med Chem 64 17063-17078 (2021)
PMID: 34806883

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-532337 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta sliding clamp Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 386 amino acids
Theoretical weight: 42.8 KDa
Source organism: Escherichia coli 2-427-07_S4_C3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:
Peptide 38 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 6 amino acids
Theoretical weight: 891 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand PEG 2 x PEG
2 modified residues:
Ligand ALC 4 x ALC
Ligand ZCL 4 x ZCL

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 139.611Å b: 86.643Å c: 153.897Å
α: 90° β: 92.56° γ: 90°
R-values:
R R work R free
0.21 0.208 0.233
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Fighting antibiotic resistance-strategies and (pre)clinical developments to find new antibacterials.
Walesch et al. (2023)
2 more