› 7azn

X-ray diffraction
2.09Å resolution

Structure of mouse AsterC (GramD1c) with a new cholesterol-derived compound

Released:
DOI: 10.2210/pdb7azn/pdb
Model geometry
Fit model/data
PDB entry 7azn coloured by chain, front view.
PDB entry 7azn coloured by chain, side view.
PDB entry 7azn coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Selective Aster inhibitors distinguish vesicular and nonvesicular sterol transport mechanisms.
Xiao X, Kim Y, Romartinez-Alonso B, Sirvydis K, Ory DS, Schwabe JWR, Jung ME, Tontonoz P
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33376205

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein Aster-C Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 20.83 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8CI52 (Residues: 327-503; Coverage: 27%)
Gene name: Gramd1c
Sequence domains: VAD1 Analog of StAR-related lipid transfer domain

Ligands and Environments

3 bound ligands:
Ligand YK8 1 x YK8
Ligand EOH 6 x EOH
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 65.995Å b: 49.397Å c: 61.691Å
α: 90° β: 100.07° γ: 90°
R-values:
R R work R free
0.219 0.217 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Sterols, Oxysterols, and Accessible Cholesterol: Signalling for Homeostasis, in Immunity and During Development.
Griffiths et al. (2021)
3 more

2 mentions without citation

Aster proteins mediate carotenoid transport in mammalian cells.
Bandara et al. (2022)
1 more

PDB-REDO

The sliders below show the change in model quality between original PDB entry and the PDB-REDO entry

Model Geometry
 
 
Data not available
DNA/RNA Geometry
 
 
Data not available
Fit model/data
 
 
Data not available
PDB-REDO