› 7b0u

Electron Microscopy
3.86Å resolution

Stressosome complex from Listeria innocua

Released:
DOI: 10.2210/pdb7b0u/pdb
PDB entry 7b0u coloured by chain, front view.
PDB entry 7b0u coloured by chain, side view.
PDB entry 7b0u coloured by chain, top view.
Source organism: Listeria innocua Clip11262
Primary publication:
Molecular insights into intra-complex signal transmission during stressosome activation.
Miksys A, Fu L, Madej MG, Guerreiro DN, Kaltwasser S, Conway M, Ejder S, Bruckmann A, Marles-Wright J, Lewis RJ, O'Byrne C, Pané-Farré J, Ziegler C
Commun Biol 5 621 (2022)
PMID: 35760945
Related structures: EMD-11971

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 60-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181344 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
STAS domain-containing protein Chains: 7, A, C, G, K, Q, R, V, d, e, g, k, s, t, v, z
Molecule details ›
Chains: 7, A, C, G, K, Q, R, V, d, e, g, k, s, t, v, z
Length: 278 amino acids
Theoretical weight: 31.7 KDa
Source organism: Listeria innocua Clip11262
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92DC6 (Residues: 1-278; Coverage: 100%)
Gene name: RsbR
Sequence domains:
STAS domain-containing protein Chains: 0, 2, B, D, H, J, L, S, W, Y, f, h, l, n, u, w
Molecule details ›
Chains: 0, 2, B, D, H, J, L, S, W, Y, f, h, l, n, u, w
Length: 278 amino acids
Theoretical weight: 31.78 KDa
Source organism: Listeria innocua Clip11262
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92DC6 (Residues: 1-278; Coverage: 100%)
Gene name: RsbR
Sequence domains:
STAS domain-containing protein Chains: 1, 4, 5, E, F, I, N, O, T, U, X, a, b, i, j, m, p, q, x, y
Molecule details ›
Chains: 1, 4, 5, E, F, I, N, O, T, U, X, a, b, i, j, m, p, q, x, y
Length: 118 amino acids
Theoretical weight: 12.61 KDa
Source organism: Listeria innocua Clip11262
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7AP18 (Residues: 1-118; Coverage: 100%)
Gene name: rsbS
Sequence domains: STAS domain
STAS domain-containing protein Chains: 3, M, Z, o
Molecule details ›
Chains: 3, M, Z, o
Length: 278 amino acids
Theoretical weight: 31.86 KDa
Source organism: Listeria innocua Clip11262
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92DC6 (Residues: 1-278; Coverage: 100%)
Gene name: RsbR
Sequence domains:
STAS domain-containing protein Chains: 6, P, c, r
Molecule details ›
Chains: 6, P, c, r
Length: 278 amino acids
Theoretical weight: 31.78 KDa
Source organism: Listeria innocua Clip11262
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q92DC6 (Residues: 1-278; Coverage: 100%)
Gene name: RsbR
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 28 x TPO

Experiments and Validation Details

Entry percentile scores
Resolution: 3.86Å
Relevant EMDB volumes: EMD-11971
Expression system: Escherichia coli BL21

Quick links

Citations

1 citations in other articles

Computational and cellular exploration of the protein-protein interaction between Vibrio fischeri STAS domain protein SypA and serine kinase SypE.
Milton et al. (2023)

1 mention without citation

Molecular insights into intra-complex signal transmission during stressosome activation.
Miksys et al. (2022)