› 7b1w

X-ray diffraction
1.94Å resolution

Crystal structure of plastidial ribulose epimerase RPE1 from the model alga Chlamydomonas reinhardtii

Released:
DOI: 10.2210/pdb7b1w/pdb
PDB entry 7b1w coloured by chain, front view.
PDB entry 7b1w coloured by chain, side view.
PDB entry 7b1w coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Characterization of chloroplast ribulose-5-phosphate-3-epimerase from the microalga Chlamydomonas reinhardtii.
Meloni M, Fanti S, Tedesco D, Gurrieri L, Trost P, Fermani S, Lemaire SD, Zaffagnini M, Henri J
Plant Physiol (2023)
PMID: 38134324

Function and Biology Details

Reaction catalysed: 
D-ribulose 5-phosphate = D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108679 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribulose-phosphate 3-epimerase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 247 amino acids
Theoretical weight: 26.81 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8IKW6 (Residues: 28-265; Coverage: 90%)
Gene name: CHLRE_12g511900v5
Sequence domains: Ribulose-phosphate 3 epimerase family

Ligands and Environments

1 bound ligand:
Ligand ZN 12 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 77.293Å b: 297.322Å c: 77.747Å
α: 90° β: 116.3° γ: 90°
R-values:
R R work R free
0.185 0.184 0.214
Expression system: Escherichia coli BL21(DE3)

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