› 7b5u

X-ray diffraction
1.2Å resolution

RCK_C domain dimer of S.agalactiae BusR in complex with c-di-AMP

Released:
DOI: 10.2210/pdb7b5u/pdb
PDB entry 7b5u coloured by chain, front view.
PDB entry 7b5u coloured by chain, side view.
PDB entry 7b5u coloured by chain, top view.
Source organism: Streptococcus agalactiae
Primary publication:
BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture.
Bandera AM, Bartho J, Lammens K, Drexler DJ, Kleinschwärzer J, Hopfner KP, Witte G
OpenAccess logo Nucleic Acids Res 49 10166-10177 (2021)
PMID: 34432045

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GntR family transcriptional regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 81 amino acids
Theoretical weight: 9.01 KDa
Source organism: Streptococcus agalactiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K0JNC6 (Residues: 134-213; Coverage: 38%)
Gene names: AX245_01365, BM110_ORF1201, C4618_04280, C6N07_09340, QP229_09620, WA45_04195
Sequence domains: TrkA-C domain

Ligands and Environments

1 bound ligand:
Ligand 2BA 1 x 2BA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P21
Unit cell:
a: 33.164Å b: 60.947Å c: 35.714Å
α: 90° β: 101.73° γ: 90°
R-values:
R R work R free
0.132 0.131 0.155
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Recent advances and perspectives in nucleotide second messenger signaling in bacteria.
Hengge et al. (2023)
4 more

1 mention without citation

BusR senses bipartite DNA binding motifs by a unique molecular ruler architecture.
Bandera et al. (2021)