› 7b6d

Electron Microscopy
4.27Å resolution

Drosophila melanogaster TRAPPCore (C1, C2, C2L, C3a/b, C4, C5, C6 subunits)

Released:
DOI: 10.2210/pdb7b6d/pdb
PDB entry 7b6d coloured by chain, front view.
PDB entry 7b6d coloured by chain, side view.
PDB entry 7b6d coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Cryo-EM structure of metazoan TRAPPIII, the multi-subunit complex that activates the GTPase Rab1.
Galindo A, Planelles-Herrero VJ, Degliesposti G, Munro S
OpenAccess logo EMBO J 40 e107608 (2021)
PMID: 34018214
Related structures: EMD-12052

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-106776 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Trafficking protein particle complex subunit Chains: A, G
Molecule details ›
Chains: A, G
Length: 200 amino acids
Theoretical weight: 22.9 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q9VSY8 (Residues: 1-178; Coverage: 100%)
Gene names: BET3, Bet3, Bet3p, CG3911, Dmel\CG3911, Dmel_CG3911, TRAPPC3, dBet3
Sequence domains: Transport protein particle (TRAPP) component
GEO08327p1 Chain: B
Molecule details ›
Chain: B
Length: 152 amino acids
Theoretical weight: 17.6 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q9VF82 (Residues: 1-152; Coverage: 100%)
Gene names: BcDNA:RH37427, CG6196, Dmel\CG6196, Dmel_CG6196, TRAPPC6, Trs33, Trs33p, dTrs33
Sequence domains: Transport protein particle (TRAPP) component
Trafficking protein particle complex subunit Chain: C
Molecule details ›
Chain: C
Length: 145 amino acids
Theoretical weight: 16.99 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q9VA95 (Residues: 1-145; Coverage: 100%)
Gene names: BET5, Bet5, Bet5p, CG1359, CG1359-RA, Dmel\CG1359, Dmel_CG1359, TRAPPC1
Sequence domains: Sybindin-like family
Trafficking protein particle complex subunit Chain: D
Molecule details ›
Chain: D
Length: 219 amino acids
Theoretical weight: 24.74 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q9VLI9 (Residues: 1-219; Coverage: 100%)
Gene names: CG9298, CG9298-RA, Dmel\CG9298, Dmel_CG9298, TRAPPC4, TRS23, Trs23, Trs23p
Sequence domains: Sybindin-like family
Probable trafficking protein particle complex subunit 2 Chain: E
Molecule details ›
Chain: E
Length: 139 amino acids
Theoretical weight: 16.67 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q9VUZ1 (Residues: 1-139; Coverage: 100%)
Gene names: CG5161, Trs20, l(3)72Dh
Sequence domains: Sedlin, N-terminal conserved region
Trafficking protein particle complex subunit 5 Chain: F
Molecule details ›
Chain: F
Length: 194 amino acids
Theoretical weight: 22.37 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: Q7K2Q8 (Residues: 1-194; Coverage: 100%)
Gene names: CG10153, Dmel\CG10153, Dmel_CG10153, TRAPPC5, Trs31, Trs31p
Sequence domains: Transport protein particle (TRAPP) component
Trafficking protein particle complex subunit 2-like protein Chain: H
Molecule details ›
Chain: H
Length: 138 amino acids
Theoretical weight: 15.51 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: A1Z8I0 (Residues: 1-138; Coverage: 100%)
Gene names: CG9067, Dmel\CG9067, Dmel_CG9067, TRAPPC2L, Tca17
Sequence domains: Sedlin, N-terminal conserved region

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.27Å
Relevant EMDB volumes: EMD-12052
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017

Quick links

Citations

4 review citations

Who's in control? Principles of Rab GTPase activation in endolysosomal membrane trafficking and beyond.
Borchers et al. (2021)
3 more

1 mention without citation

Cryo-EM structure of metazoan TRAPPIII, the multi-subunit complex that activates the GTPase Rab1.
Galindo et al. (2021)