› 7baz

X-ray diffraction
1.3Å resolution

Lysozyme crystallized in the presence of the hydrated deep eutectic solvent Choline chloride-Glycerol 1:2

Released:
DOI: 10.2210/pdb7baz/pdb
PDB entry 7baz coloured by chain, front view.
PDB entry 7baz coloured by chain, side view.
PDB entry 7baz coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Introducing Protein Crystallization in Hydrated Deep Eutectic Solvents
Belviso BD, Perna FM, Carrozzini B, Trotta M, Capriati V, Caliandro R
Acs Sustain Chem Eng 9 8435-8449 (2021)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family

Ligands and Environments

4 bound ligands:
Ligand ACT 1 x ACT
Ligand CHT 2 x CHT
Ligand CL 13 x CL
Ligand NA 5 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 78.901Å b: 78.901Å c: 37.124Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.127 0.164

Quick links