› 7bbu

X-ray diffraction
2.19Å resolution

Crystal Structure of human Prolyl-tRNA synthetase in complex with NCP26 and L-Proline

Released:
DOI: 10.2210/pdb7bbu/pdb
PDB entry 7bbu coloured by chain, front view.
PDB entry 7bbu coloured by chain, side view.
PDB entry 7bbu coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Johansson C, Tye M, Payne NC, Mazitschek R, Krojer T, Oppermann UCT

Function and Biology Details

Reactions catalysed: 
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-139771 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional glutamate/proline--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 515 amino acids
Theoretical weight: 58.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07814 (Residues: 1000-1512; Coverage: 34%)
Gene names: EPRS, EPRS1, GLNS, PARS, PIG32, QARS, QPRS
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand PRO 1 x PRO
Ligand MU5 1 x MU5
Ligand EDO 10 x EDO
Ligand NO3 1 x NO3
Ligand ZN 1 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 87.09Å b: 87.09Å c: 108.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.206 0.233
Expression system: Escherichia coli BL21(DE3)

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