› 7bh1

Electron Microscopy
3.38Å resolution

Cryo-EM Structure of KdpFABC in E1 state with K

Released:
DOI: 10.2210/pdb7bh1/pdb
PDB entry 7bh1 coloured by chain, front view.
PDB entry 7bh1 coloured by chain, side view.
PDB entry 7bh1 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis for potassium transport in prokaryotes by KdpFABC.
Sweet ME, Larsen C, Zhang X, Schlame M, Pedersen BP, Stokes DL
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34272288
Related structures: EMD-12185

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + K(+)(Side 1) = ADP + phosphate + K(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137301 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Potassium-transporting ATPase potassium-binding subunit Chain: A
Molecule details ›
Chain: A
Length: 557 amino acids
Theoretical weight: 59.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03959 (Residues: 1-557; Coverage: 100%)
Gene names: JW0686, b0698, kdpA
Sequence domains: Potassium-transporting ATPase A subunit
Potassium-transporting ATPase ATP-binding subunit Chain: B
Molecule details ›
Chain: B
Length: 682 amino acids
Theoretical weight: 72.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03960 (Residues: 1-682; Coverage: 100%)
Gene names: JW0685, b0697, kdpB
Sequence domains:
Potassium-transporting ATPase KdpC subunit Chain: C
Molecule details ›
Chain: C
Length: 208 amino acids
Theoretical weight: 22.3 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03961 (Residues: 1-190; Coverage: 100%)
Gene names: JW0684, b0696, kdpC
Sequence domains: K+-transporting ATPase, c chain
Potassium-transporting ATPase KdpF subunit Chain: D
Molecule details ›
Chain: D
Length: 29 amino acids
Theoretical weight: 3.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P36937 (Residues: 1-29; Coverage: 100%)
Gene names: JW0687, b4513, kdpF
Sequence domains: F subunit of K+-transporting ATPase (Potass_KdpF)

Ligands and Environments

2 bound ligands:
Ligand K 1 x K
Ligand 9Y0 1 x 9Y0
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.38Å
Relevant EMDB volumes: EMD-12185
Expression system: Escherichia coli

Quick links

Citations

2 review citations

P-type ATPases: Many more enigmas left to solve.
Palmgren M. (2023)
1 more

1 mention without citation

Inhibited KdpFABC transitions into an E1 off-cycle state.
Silberberg et al. (2022)