› 7bhc

X-ray diffraction
1.87Å resolution

Escherichia coli YtfE E125L

Released:
DOI: 10.2210/pdb7bhc/pdb
PDB entry 7bhc coloured by chain, front view.
PDB entry 7bhc coloured by chain, side view.
PDB entry 7bhc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural Basis of RICs Iron Donation for Iron-Sulfur Cluster Biogenesis.
Silva LSO, Matias PM, Romão CV, Saraiva LM
OpenAccess logo Front Microbiol 12 670681 (2021)
PMID: 33995335

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-sulfur cluster repair protein YtfE Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.7 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P69506 (Residues: 2-220; Coverage: 100%)
Gene names: JW4167, b4209, ytfE
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand FE 4 x FE
Ligand O 2 x O
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 59.79Å b: 49.608Å c: 87.819Å
α: 90° β: 100.143° γ: 90°
R-values:
R R work R free
0.184 0.182 0.211
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 review citation

Repair of Iron Center Proteins-A Different Class of Hemerythrin-like Proteins.
Silva et al. (2022)
1 other article