› 7bhd

X-ray diffraction
1.4Å resolution

FimH in complex with alpha1,6 core-fucosylated oligomannose-3, crystallized in the trigonal space group

Released:
DOI: 10.2210/pdb7bhd/pdb
PDB entry 7bhd coloured by chain, front view.
PDB entry 7bhd coloured by chain, side view.
PDB entry 7bhd coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural insights into a cooperative switch between one and two FimH bacterial adhesins binding pauci- and high-mannose type N-glycan receptors.
Krammer EM, Bridot C, Serna S, Echeverria B, Semwal S, Roubinet B, van Noort K, Wilbers RP, Bourenkov G, de Ruyck J, Landemarre L, Reichardt N, Bouckaert J
OpenAccess logo J Biol Chem 104627 (2023)
PMID: 36944399

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Type 1 fimbrin D-mannose specific adhesin Chains: A, B
Molecule details ›
Chains: A, B
Length: 158 amino acids
Theoretical weight: 16.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08191 (Residues: 22-179; Coverage: 57%)
Gene names: JW4283, b4320, fimH
Sequence domains: FimH, mannose binding

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
2 bound ligands:
Ligand NI 2 x NI
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P3121
Unit cell:
a: 91.21Å b: 91.21Å c: 79.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.171 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Revisiting the immunopathology of congenital disorders of glycosylation: an updated review.
Pascoal et al. (2024)
1 other article

1 mention without citation

Structural insights into a cooperative switch between one and two FimH bacterial adhesins binding pauci- and high-mannose type N-glycan receptors.
Krammer et al. (2023)