7bhe

X-ray diffraction
2.3Å resolution

DARPin_D5/Her3 domain 4 complex, monoclinic crystals

Released:
Primary publication:
Crystal structures of HER3 extracellular domain 4 in complex with the designed ankyrin-repeat protein D5.
OpenAccess logo Acta Crystallogr F Struct Biol Commun 77 192-201 (2021)
PMID: 34196609

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-149448 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DARPin_D5 Chains: A, C
Molecule details ›
Chains: A, C
Length: 138 amino acids
Theoretical weight: 14.91 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Receptor tyrosine-protein kinase erbB-3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 150 amino acids
Theoretical weight: 16.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21860 (Residues: 500-643; Coverage: 11%)
Gene names: ERBB3, HER3
Sequence domains: Growth factor receptor domain IV

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 64.872Å b: 62.254Å c: 74.545Å
α: 90° β: 102.41° γ: 90°
R-values:
R R work R free
0.187 0.184 0.254
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda