Structure analysis

DARPin_D5/Her3 domain 4 complex, orthorhombic crystals

X-ray diffraction
1.997Å resolution
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dimer
Accessible surface area: 12978.92 Å2
Buried surface area: 1937.57 Å2
Dissociation area: 871.24 Å2
Dissociation energy (ΔGdiss): 2.03 kcal/mol
Dissociation entropy (TΔSdiss): 11.41 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-539961
Assembly 2
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Multimeric state: hetero dimer
Accessible surface area: 13014.06 Å2
Buried surface area: 1915.64 Å2
Dissociation area: 893.91 Å2
Dissociation energy (ΔGdiss): 2.5 kcal/mol
Dissociation entropy (TΔSdiss): 11.41 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-539961

Macromolecules

Chains: A, C
Length: 138 amino acids
Theoretical weight: 14.91 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
InterPro:

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Chains: B, D
Length: 150 amino acids
Theoretical weight: 16.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21860 (Residues: 500-630; Coverage: 10%)
Gene names: ERBB3, HER3
Pfam: Growth factor receptor domain IV
InterPro:

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