› 7bhx

X-ray diffraction
1.08Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-542368 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase isoform type-2 Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 43.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31153 (Residues: 1-395; Coverage: 100%)
Gene names: AMS2, MAT2A, MATA2
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand DMS 1 x DMS
Ligand TO5 1 x TO5
Ligand SAM 1 x SAM
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I222
Unit cell:
a: 68.044Å b: 94.026Å c: 117.054Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.172
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Targeting allosteric regulation of cancer metabolism.
Kremer et al. (2022)
3 more