› 7bid

X-ray diffraction
1.8Å resolution

Crystal structure of v31WRAP-T, a 7-bladed designer protein

Released:
DOI: 10.2210/pdb7bid/pdb
PDB entry 7bid coloured by chain, front view.
PDB entry 7bid coloured by chain, side view.
PDB entry 7bid coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structure and stability of the designer protein WRAP-T and its permutants.
Mylemans B, Lee XY, Laier I, Helsen C, Voet ARD
OpenAccess logo Sci Rep 11 18867 (2021)
PMID: 34552189

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WD-40 repeat protein Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 30.35 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: B2J0I0 (Residues: 642-928; Coverage: 23%)
Gene name: Npun_R6612
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 44.636Å b: 61.396Å c: 99.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.183
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Circular permutation at azurin's active site slows down its folding.
Das et al. (2023)

1 mention without citation

Structure and stability of the designer protein WRAP-T and its permutants.
Mylemans et al. (2021)