› 7bkd

Electron Microscopy
3Å resolution

Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from Methanospirillum hungatei (heterodislfide reductase core and mobile arm in conformational state 1, composite structure)

Released:
DOI: 10.2210/pdb7bkd/pdb
PDB entry 7bkd coloured by chain, front view.
PDB entry 7bkd coloured by chain, side view.
PDB entry 7bkd coloured by chain, top view.
Source organism: Methanospirillum hungatei JF-1
Primary publication:
Three-megadalton complex of methanogenic electron-bifurcating and CO2-fixing enzymes.
Watanabe T, Pfeil-Gardiner O, Kahnt J, Koch J, Shima S, Murphy BJ
Science 373 1151-1156 (2021)
PMID: 34516836
Related structures: EMD-12210

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-173402 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
CoB--CoM heterodisulfide reductase iron-sulfur subunit A Chains: A, a
Molecule details ›
Chains: A, a
Length: 671 amino acids
Theoretical weight: 72.89 KDa
Source organism: Methanospirillum hungatei JF-1
UniProt:
  • Canonical: Q2FKZ1 (Residues: 1-671; Coverage: 100%)
Gene name: Mhun_1838
Sequence domains:
4Fe-4S ferredoxin-type domain-containing protein Chains: C, c
Molecule details ›
Chains: C, c
Length: 191 amino acids
Theoretical weight: 21.71 KDa
Source organism: Methanospirillum hungatei JF-1
UniProt:
  • Canonical: Q2FKZ3 (Residues: 1-191; Coverage: 100%)
Gene name: Mhun_1836
Sequence domains: 4Fe-4S dicluster domain
Cysteine-rich domain-containing protein Chains: B, b
Molecule details ›
Chains: B, b
Length: 296 amino acids
Theoretical weight: 32.93 KDa
Source organism: Methanospirillum hungatei JF-1
UniProt:
  • Canonical: Q2FKZ2 (Residues: 1-296; Coverage: 100%)
Gene name: Mhun_1837
Sequence domains: Cysteine-rich domain
F420-non-reducing hydrogenase iron-sulfur subunit D domain-containing protein Chain: F
Molecule details ›
Chain: F
Length: 140 amino acids
Theoretical weight: 15.69 KDa
Source organism: Methanospirillum hungatei JF-1
UniProt:
  • Canonical: Q2FKZ0 (Residues: 1-140; Coverage: 100%)
Gene name: Mhun_1839
Sequence domains: Methyl-viologen-reducing hydrogenase, delta subunit
4Fe-4S ferredoxin-type domain-containing protein Chain: E
Molecule details ›
Chain: E
Length: 414 amino acids
Theoretical weight: 45.64 KDa
Source organism: Methanospirillum hungatei JF-1
UniProt:
  • Canonical: Q2FME3 (Residues: 1-414; Coverage: 100%)
Gene name: Mhun_2020
Sequence domains:
4Fe-4S Mo/W bis-MGD-type domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 686 amino acids
Theoretical weight: 75.91 KDa
Source organism: Methanospirillum hungatei JF-1
UniProt:
  • Canonical: Q2FRK1 (Residues: 1-686; Coverage: 100%)
Gene name: Mhun_2021
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand SF4 18 x SF4
Ligand FAD 3 x FAD
Ligand 9S8 4 x 9S8
Ligand FES 1 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-12210

Quick links

Citations

4 review citations

Evolving understanding of rumen methanogen ecophysiology.
Khairunisa et al. (2023)
3 more