7bkp

Electron Microscopy
2.8Å resolution

CryoEM structure of disease related M854K MDA5-dsRNA filament in complex with ATP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-185919 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Interferon-induced helicase C domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 1025 amino acids
Theoretical weight: 116.12 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8R5F7 (Residues: 1-1025; Coverage: 100%)
Gene name: Ifih1
Sequence domains:
RNA (5'-R(P*CP*UP*CP*UP*CP*CP*UP*CP*GP*GP*CP*UP*UP*G)-3') Chain: Z
Molecule details ›
Chain: Z
Length: 14 nucleotides
Theoretical weight: 4.35 KDa
Source organism: Pseudomonas virus phi6
Expression system: Not provided
RNA (5'-R(P*CP*AP*AP*GP*CP*CP*GP*AP*GP*GP*AP*GP*AP*G)-3') Chain: X
Molecule details ›
Chain: X
Length: 14 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Pseudomonas virus phi6
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.8Å
Relevant EMDB volumes: EMD-12213
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided