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Architecture and mechanism of metazoan retromer:SNX3 tubular coat assembly.

<div class='caption-title'>Overview of metazoan and fungal retromer:SNX3 coats assembled on membranes in the presence of cargo peptides.</div>
<div class='caption-title'>Structure of the membrane-proximal region of the metazoan retromer:SNX3 complex.</div>
<div class='caption-title'>Conserved, asymmetric VPS35 dimerization interface.</div>
Leneva N, Kovtun O, Morado DR, Briggs JAG, Owen DJ
OpenAccess logo Sci Adv 7 (2021)
Related entries: 7bln, 7blp, 7blq, 7blr

Cited: 29 times
EuropePMC logo PMID: 33762348

Abstract

Retromer is a master regulator of cargo retrieval from endosomes, which is critical for many cellular processes including signaling, immunity, neuroprotection, and virus infection. The retromer core (VPS26/VPS29/VPS35) is present on cargo-transporting, tubular carriers along with a range of sorting nexins. Here, we elucidate the structural basis of membrane tubulation and coupled cargo recognition by metazoan and fungal retromer coats assembled with the non-Bin1/Amphiphysin/Rvs (BAR) sorting nexin SNX3 using cryo-electron tomography. The retromer core retains its arched, scaffolding structure but changes its mode of membrane recruitment when assembled with different SNX adaptors, allowing cargo recognition at subunit interfaces. Thus, membrane bending and cargo incorporation can be modulated to allow retromer to traffic cargoes along different cellular transport routes.

Reviews citing this publication (11)

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  2. Parkinson's Disease and SARS-CoV-2 Infection: Particularities of Molecular and Cellular Mechanisms Regarding Pathogenesis and Treatment. Anghelescu A, Onose G, Popescu C, Băilă M, Stoica SI, Postoiu R, Brumă E, Petcu IR, Ciobanu V, Munteanu C. Biomedicines 10 1000 (2022)
  3. Out of the ESCPE room: Emerging roles of endosomal SNX-BARs in receptor transport and host-pathogen interaction. Simonetti B, Daly JL, Cullen PJ. Traffic 24 234-250 (2023)
  4. Who's in control? Principles of Rab GTPase activation in endolysosomal membrane trafficking and beyond. Borchers AC, Langemeyer L, Ungermann C. J Cell Biol 220 e202105120 (2021)
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  6. Transmembrane Membrane Readers form a Novel Class of Proteins That Include Peripheral Phosphoinositide Recognition Domains and Viral Spikes. Overduin M, Tran A, Eekels DM, Overduin F, Kervin TA. Membranes (Basel) 12 1161 (2022)
  7. An Update on Coat Protein Complexes for Vesicle Formation in Plant Post-Golgi Trafficking. Law KC, Chung KK, Zhuang X. Front Plant Sci 13 826007 (2022)
  8. An evolving understanding of sorting signals for endosomal retrieval. Yong X, Mao L, Seaman MNJ, Jia D. iScience 25 104254 (2022)
  9. Deliver on Time or Pay the Fine: Scheduling in Membrane Trafficking. Placidi G, Campa CC. Int J Mol Sci 22 11773 (2021)
  10. Retromer dependent changes in cellular homeostasis and Parkinson's disease. Yang Z, Li Z, Teasdale RD. Essays Biochem 65 987-998 (2021)
  11. The role of Evi/Wntless in exporting Wnt proteins. Wolf L, Boutros M. Development 150 dev201352 (2023)

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