› 7bmv

X-ray diffraction
1.9Å resolution

p62PH in cesium chloride (0.25 M CsCl in protein buffer and cryo protectant)

Released:
DOI: 10.2210/pdb7bmv/pdb
PDB entry 7bmv coloured by chain, front view.
PDB entry 7bmv coloured by chain, side view.
PDB entry 7bmv coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Cesium based phasing of macromolecules: a general easy to use approach for solving the phase problem.
Koelmel W, Kuper J, Kisker C
OpenAccess logo Sci Rep 11 17038 (2021)
PMID: 34426585

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BSD domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.18 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0RZ08 (Residues: 1-109; Coverage: 16%)
Gene name: CTHT_0001260
Sequence domains: TFIIH p62 subunit, N-terminal domain

Ligands and Environments

2 bound ligands:
Ligand CS 2 x CS
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 39.74Å b: 102.3Å c: 75.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.223
Expression system: Escherichia coli

Quick links

Citations

1 review citation

SARS-CoV-2 proteins structural studies using synchrotron radiation.
Kosenko et al. (2023)