› 7bnq

Electron Microscopy
4.1Å resolution

Lateral-closed conformation of the lid-locked BAM complex (BamA E435C S665C, BamBDCE) by cryoEM

Released:
DOI: 10.2210/pdb7bnq/pdb
PDB entry 7bnq coloured by chain, front view.
PDB entry 7bnq coloured by chain, side view.
PDB entry 7bnq coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The role of membrane destabilisation and protein dynamics in BAM catalysed OMP folding.
White P, Haysom SF, Iadanza MG, Higgins AJ, Machin JM, Whitehouse JM, Horne JE, Schiffrin B, Carpenter-Platt C, Calabrese AN, Storek KM, Rutherford ST, Brockwell DJ, Ranson NA, Radford SE
OpenAccess logo Nat Commun 12 4174 (2021)
PMID: 34234105
Related structures: EMD-12232

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-141767 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 810 amino acids
Theoretical weight: 90.6 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A940 (Residues: 1-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 392 amino acids
Theoretical weight: 41.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77774 (Residues: 1-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Outer membrane protein assembly factor BamD Chain: D
Outer membrane protein assembly factor BamE Chain: E

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-12232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Targeting BAM for Novel Therapeutics against Pathogenic Gram-Negative Bacteria.
Overly Cottom et al. (2023)
2 more