7bt6

Electron Microscopy
3.12Å resolution

Cryo-EM structure of pre-60S ribosome from Saccharomyces cerevisiae rpl4delta63-87 strain at 3.12 Angstroms resolution(state R1)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 47-mer (preferred)
PDBe Complex ID:
PDB-CPX-135797 (preferred)
Entry contents:
44 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (47 distinct):
Large ribosomal subunit protein uL2A Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 27.46 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P0CX45 (Residues: 1-254; Coverage: 100%)
Gene names: RPL2A, RPL5B, YFR031BC, YFR031C-A
Sequence domains:
Large ribosomal subunit protein uL3 Chain: B
Molecule details ›
Chain: B
Length: 387 amino acids
Theoretical weight: 43.85 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P14126 (Residues: 1-387; Coverage: 100%)
Gene names: MAK8, RPL3, TCM1, YOR063W, YOR29-14
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4A Chain: C
Large ribosomal subunit protein uL18 Chain: D
Large ribosomal subunit protein eL6A Chain: E
Large ribosomal subunit protein uL30A Chain: F
Large ribosomal subunit protein eL8A Chain: G
Large ribosomal subunit protein uL6A Chain: H
Large ribosomal subunit protein uL5A Chain: J
Large ribosomal subunit protein eL13A Chain: L
Large ribosomal subunit protein eL14A Chain: M
Large ribosomal subunit protein eL15A Chain: N
Large ribosomal subunit protein uL13A Chain: O
Large ribosomal subunit protein uL22A Chain: P
Large ribosomal subunit protein eL18A Chain: Q
Large ribosomal subunit protein eL19A Chain: R
Large ribosomal subunit protein eL20A Chain: S
Large ribosomal subunit protein eL21A Chain: T
Large ribosomal subunit protein eL22A Chain: U
Large ribosomal subunit protein uL14A Chain: V
Ribosome assembly factor MRT4 Chain: W
Large ribosomal subunit protein uL23 Chain: X
Large ribosomal subunit protein uL24A Chain: Y
Large ribosomal subunit protein eL27A Chain: Z
Large ribosomal subunit protein uL15 Chain: a
Nucleolar GTP-binding protein 1 Chain: b
Large ribosomal subunit protein eL30 Chain: c
Large ribosomal subunit protein eL31A Chain: d
Large ribosomal subunit protein eL32 Chain: e
Large ribosomal subunit protein eL33A Chain: f
Large ribosomal subunit protein eL34A Chain: g
Large ribosomal subunit protein uL29A Chain: h
Large ribosomal subunit protein eL36A Chain: i
Large ribosomal subunit protein eL37A Chain: j
Large ribosomal subunit protein eL38 Chain: k
Nucleolar GTP-binding protein 2 Chain: m
Large ribosomal subunit protein eL43A Chain: p
Ribosome biogenesis protein NSA2 Chain: r
Ribosome biogenesis protein RLP24 Chain: u
Ribosome biogenesis protein RPF2 Chain: v
Regulator of ribosome biosynthesis Chain: w
Ribosome assembly protein 4 Chain: x
Eukaryotic translation initiation factor 6 Chain: y
UPF0642 protein YBL028C Chain: z
RDN25-1 rRNA Chain: 1
RDN5.8-1 rRNA Chain: 2
RDN5-2 rRNA Chain: 3

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.12Å
Relevant EMDB volumes: EMD-30170