Function and Biology Details
Reactions catalysed:
Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- B-form DNA mimic Ocr
- S-adenosyl-L-methionine-dependent methyltransferase superfamily
- P-loop containing nucleoside triphosphate hydrolase
- Type I restriction modification DNA specificity domain
- Type I restriction modification DNA specificity domain superfamily
- Protein Ocr
- DNA methylase, N-6 adenine-specific, conserved site
- DNA methylase, adenine-specific
8 more domains
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-137263 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Type I restriction enzyme EcoR124I/EcoR124II specificity subunit
Molecule details ›
Chain: E
Length: 404 amino acids
Theoretical weight: 46.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Sequence domains: Type I restriction modification DNA specificity domain
Length: 404 amino acids
Theoretical weight: 46.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical:
P10485 (Residues: 1-404; Coverage: 100%)
Sequence domains: Type I restriction modification DNA specificity domain
Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit
Molecule details ›
Chain: A
Length: 1038 amino acids
Theoretical weight: 120.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 1038 amino acids
Theoretical weight: 120.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical: P10486 (Residues: 1-1038; Coverage: 100%)
Sequence domains:
Type I restriction enzyme EcoR124I/EcoR124II methylase subunit
Molecule details ›
Chains: B, C
Length: 520 amino acids
Theoretical weight: 58.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 520 amino acids
Theoretical weight: 58.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical: P10484 (Residues: 1-520; Coverage: 100%)
Sequence domains:
Protein Ocr
Molecule details ›
Chains: D, F
Length: 117 amino acids
Theoretical weight: 13.82 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
Sequence domains: DNA mimic ocr
Length: 117 amino acids
Theoretical weight: 13.82 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
- Canonical: P03775 (Residues: 1-117; Coverage: 100%)
Sequence domains: DNA mimic ocr
