› 7bva

X-ray diffraction
2.3Å resolution

Crystal structure of UDP-N-acetylmuramic Acid L-alanine ligase (MurC) from Mycobacterium bovis

Released:
DOI: 10.2210/pdb7bva/pdb
PDB entry 7bva coloured by chain, front view.
PDB entry 7bva coloured by chain, side view.
PDB entry 7bva coloured by chain, top view.
Source organism: Mycobacterium tuberculosis variant bovis AF2122/97
Primary publication:
Crystal structures of UDP-N-acetylmuramic acid L-alanine ligase (MurC) from Mycobacterium bovis with and without UDP-N-acetylglucosamine.
Seo PW, Park SY, Hofmann A, Kim JS
Acta Crystallogr D Struct Biol 77 618-627 (2021)
PMID: 33950018

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramate--L-alanine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 54.4 KDa
Source organism: Mycobacterium tuberculosis variant bovis AF2122/97
Expression system: Escherichia coli
UniProt:
  • Canonical: P65473 (Residues: 1-494; Coverage: 100%)
Gene names: BQ2027_MB2176C, murC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 65.3Å b: 76.703Å c: 103.962Å
α: 90° β: 105.99° γ: 90°
R-values:
R R work R free
0.207 0.206 0.228
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

In Silico Subtractive Proteomics and Molecular Docking Approaches for the Identification of Novel Inhibitors against Streptococcus pneumoniae Strain D39.
Shami et al. (2023)