› 7bvg

Electron Microscopy
3.1Å resolution

Cryo-EM structure of Mycobacterium smegmatis arabinosyltransferase EmbA-EmbB-AcpM2 in complex with di-arabinose.

Released:
DOI: 10.2210/pdb7bvg/pdb
PDB entry 7bvg coloured by chain, front view.
PDB entry 7bvg coloured by chain, side view.
PDB entry 7bvg coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structures of cell wall arabinosyltransferases with the anti-tuberculosis drug ethambutol.
Zhang L, Zhao Y, Gao Y, Wu L, Gao R, Zhang Q, Wang Y, Wu C, Wu F, Gurcha SS, Veerapen N, Batt SM, Zhao W, Qin L, Yang X, Wang M, Zhu Y, Zhang B, Bi L, Zhang X, Yang H, Guddat LW, Xu W, Wang Q, Li J, Besra GS, Rao Z
Science 368 1211-1219 (2020)
PMID: 32327601
Related structures: EMD-30219

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-106399 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Probable arabinosyltransferase B Chain: A
Molecule details ›
Chain: A
Length: 1088 amino acids
Theoretical weight: 116.56 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R613 (Residues: 1-1080; Coverage: 100%)
Gene name: MSMEG_6388
Sequence domains:
Probable arabinosyltransferase A Chain: B
Molecule details ›
Chain: B
Length: 1100 amino acids
Theoretical weight: 119.08 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R614 (Residues: 1-1082; Coverage: 100%)
Gene name: MSMEG_6389
Sequence domains:
Meromycolate extension acyl carrier protein Chain: P
Molecule details ›
Chain: P
Length: 99 amino acids
Theoretical weight: 10.74 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R0B3 (Residues: 1-99; Coverage: 100%)
Gene names: MSMEG_4326, MSMEI_4226, acpM
Sequence domains: Phosphopantetheine attachment site

Ligands and Environments

Carbohydrate polymer : NEW Components: BXY
Carbohydrate polymer
5 bound ligands:
Ligand F8L 1 x F8L
Ligand CDL 2 x CDL
Ligand CA 2 x CA
Ligand PO4 1 x PO4
Ligand PNS 1 x PNS
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-30219
Expression system: Mycolicibacterium smegmatis MC2 155

Quick links

Citations

16 review citations

Mechanisms of Drug-Induced Tolerance in Mycobacterium tuberculosis.
Goossens et al. (2020)
15 more

2 mentions without citation

Antibiotics and resistance: the two-sided coin of the mycobacterial cell wall.
Batt et al. (2020)
1 more