› 7c8o

X-ray diffraction
1.84Å resolution

Crystal structure of IscU D40A/H106A variant

Released:
DOI: 10.2210/pdb7c8o/pdb
PDB entry 7c8o coloured by chain, front view.
PDB entry 7c8o coloured by chain, side view.
PDB entry 7c8o coloured by chain, top view.
Source organism: Methanothrix thermoacetophila
Entry authors: Kunichika K, Takahashi Y, Fujishiro T

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106062 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NIF system FeS cluster assembly NifU N-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 137 amino acids
Theoretical weight: 15.15 KDa
Source organism: Methanothrix thermoacetophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0B757 (Residues: 1-129; Coverage: 100%)
Gene name: Mthe_0741
Sequence domains: NifU-like N terminal domain

Ligands and Environments

1 bound ligand:
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 120.678Å b: 60.376Å c: 64.562Å
α: 90° β: 95.78° γ: 90°
R-values:
R R work R free
0.201 0.194 0.222
Expression system: Escherichia coli BL21(DE3)

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