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Cryo-EM structure of a Ca2+-bound photosynthetic LH1-RC complex containing multiple αβ-polypeptides.

<div class='caption-title'>Overall structure and cofactor arrangement of the Trv. strain 970 LH1-RC complex.</div>
<div class='caption-title'>Ca2+- and BChl a-binding sites in the Trv. strain 970 LH1 complex.</div>
<div class='caption-title'>Arrangement of the LH1 multiple polypeptides.</div>
Tani K, Kanno R, Makino Y, Hall M, Takenouchi M, Imanishi M, Yu LJ, Overmann J, Madigan MT, Kimura Y, Mizoguchi A, Humbel BM, Wang-Otomo ZY
OpenAccess logo Nat Commun 11 4955 (2020)
Cited: 21 times
EuropePMC logo PMID: 33009385

Abstract

The light-harvesting-reaction center complex (LH1-RC) from the purple phototrophic bacterium Thiorhodovibrio strain 970 exhibits an LH1 absorption maximum at 960 nm, the most red-shifted absorption for any bacteriochlorophyll (BChl) a-containing species. Here we present a cryo-EM structure of the strain 970 LH1-RC complex at 2.82 Å resolution. The LH1 forms a closed ring structure composed of sixteen pairs of the αβ-polypeptides. Sixteen Ca ions are present in the LH1 C-terminal domain and are coordinated by residues from the αβ-polypeptides that are hydrogen-bonded to BChl a. The Ca2+-facilitated hydrogen-bonding network forms the structural basis of the unusual LH1 redshift. The structure also revealed the arrangement of multiple forms of α- and β-polypeptides in an individual LH1 ring. Such organization indicates a mechanism of interplay between the expression and assembly of the LH1 complex that is regulated through interactions with the RC subunits inside.

Articles - 7c9r mentioned but not cited (5)

  1. Cryo-EM structure of the Rhodospirillum rubrum RC-LH1 complex at 2.5 Å. Qian P, Croll TI, Swainsbury DJK, Castro-Hartmann P, Moriarty NW, Sader K, Hunter CN. Biochem J 478 3253-3263 (2021)
  2. An LH1-RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins. Tani K, Kanno R, Kurosawa K, Takaichi S, Nagashima KVP, Hall M, Yu LJ, Kimura Y, Madigan MT, Mizoguchi A, Humbel BM, Wang-Otomo ZY. Commun Biol 5 1197 (2022)
  3. research-article De novo design of energy transfer proteins housing excitonically coupled chlorophyll special pairs. Ennist N, Wang S, Kennedy M, Curti M, Sutherland G, Vasilev C, Redler R, Maffeis V, Shareef S, Sica A, Hua A, Deshmukh A, Moyer A, Hicks D, Swartz A, Cacho R, Novy N, Bera A, Kang A, Sankaran B, Johnson M, Reppert M, Ekiert D, Bhabha G, Stewart L, Caram J, Stoddard B, Romero E, Hunter CN, Baker D. Res Sq rs.3.rs-2736786 (2023)
  4. New insights on the photocomplex of Roseiflexus castenholzii revealed from comparisons of native and carotenoid-depleted complexes. Qi CH, Wang GL, Wang FF, Xin Y, Zou MJ, Madigan MT, Wang-Otomo ZY, Ma F, Yu LJ. J Biol Chem 299 105057 (2023)
  5. Structures of Rhodopseudomonas palustris RC-LH1 complexes with open or closed quinone channels. Swainsbury DJK, Qian P, Jackson PJ, Faries KM, Niedzwiedzki DM, Martin EC, Farmer DA, Malone LA, Thompson RF, Ranson NA, Canniffe DP, Dickman MJ, Holten D, Kirmaier C, Hitchcock A, Hunter CN. Sci Adv 7 (2021)


Articles citing this publication (16)

  1. Cryo-EM structure of the photosynthetic RC-LH1-PufX supercomplex at 2.8-Å resolution. Bracun L, Yamagata A, Christianson BM, Terada T, Canniffe DP, Shirouzu M, Liu LN. Sci Adv 7 eabf8864 (2021)
  2. A Ca2+-binding motif underlies the unusual properties of certain photosynthetic bacterial core light-harvesting complexes. Tani K, Kobayashi K, Hosogi N, Ji XC, Nagashima S, Nagashima KVP, Izumida A, Inoue K, Tsukatani Y, Kanno R, Hall M, Yu LJ, Ishikawa I, Okura Y, Madigan MT, Mizoguchi A, Humbel BM, Kimura Y, Wang-Otomo ZY. J Biol Chem 298 101967 (2022)
  3. A previously unrecognized membrane protein in the Rhodobacter sphaeroides LH1-RC photocomplex. Tani K, Nagashima KVP, Kanno R, Kawamura S, Kikuchi R, Hall M, Yu LJ, Kimura Y, Madigan MT, Mizoguchi A, Humbel BM, Wang-Otomo ZY. Nat Commun 12 6300 (2021)
  4. 2.4-Å structure of the double-ring Gemmatimonas phototrophica photosystem. Qian P, Gardiner AT, Šímová I, Naydenova K, Croll TI, Jackson PJ, Nupur, Kloz M, Čubáková P, Kuzma M, Zeng Y, Castro-Hartmann P, van Knippenberg B, Goldie KN, Kaftan D, Hrouzek P, Hájek J, Agirre J, Siebert CA, Bína D, Sader K, Stahlberg H, Sobotka R, Russo CJ, Polívka T, Hunter CN, Koblížek M. Sci Adv 8 eabk3139 (2022)
  5. Thiorhodovibrio frisius and Trv. litoralis spp. nov., Two Novel Members from a Clade of Fastidious Purple Sulfur Bacteria That Exhibit Unique Red-Shifted Light-Harvesting Capabilities. Methner A, Kuzyk SB, Petersen J, Bauer S, Brinkmann H, Sichau K, Wanner G, Wolf J, Neumann-Schaal M, Henke P, Tank M, Spröer C, Bunk B, Overmann J. Microorganisms 11 2394 (2023)
  6. Atomic force microscopic analysis of the light-harvesting complex 2 from purple photosynthetic bacterium Thermochromatium tepidum. Morimoto M, Hirao H, Kondo M, Dewa T, Kimura Y, Wang-Otomo ZY, Asakawa H, Saga Y. Photosynth Res (2023)
  7. Circular dichroism and resonance Raman spectroscopies of bacteriochlorophyll b-containing LH1-RC complexes. Kimura Y, Yamashita T, Seto R, Imanishi M, Honda M, Nakagawa S, Saga Y, Takenaka S, Yu LJ, Madigan MT, Wang-Otomo ZY. Photosynth Res 148 77-86 (2021)
  8. Dominant role of excitons in photosynthetic color-tuning and light-harvesting. Timpmann K, Rätsep M, Freiberg A. Front Chem 11 1231431 (2023)
  9. Electronic Structure Effects Related to the Origin of the Remarkable Near-Infrared Absorption of Blastochloris viridis' Light Harvesting 1-Reaction Center Complex. Mondragón-Solórzano G, Sandoval-Lira J, Nochebuena J, Cisneros GA, Barroso-Flores J. J Chem Theory Comput 18 4555-4564 (2022)
  10. Enhancing solar spectrum utilization in photosynthesis: exploring exciton and site energy shifts as key mechanisms. Timpmann K, Rätsep M, Freiberg A. Sci Rep 13 22299 (2023)
  11. Exciton Origin of Color-Tuning in Ca2+-Binding Photosynthetic Bacteria. Timpmann K, Rätsep M, Kangur L, Lehtmets A, Wang-Otomo ZY, Freiberg A. Int J Mol Sci 22 7338 (2021)
  12. Photosynthesis in the near infrared: the γ subunit of Blastochloris viridis LH1 red-shifts absorption beyond 1000 nm. Hitchcock A, Swainsbury DJK, Hunter CN. Biochem J 480 455-460 (2023)
  13. Salt- and pH-Dependent Thermal Stability of Photocomplexes from Extremophilic Bacteriochlorophyll b-Containing Halorhodospira Species. Kimura Y, Nakata K, Nojima S, Takenaka S, Madigan MT, Wang-Otomo ZY. Microorganisms 10 959 (2022)
  14. Structural basis for the assembly and quinone transport mechanisms of the dimeric photosynthetic RC-LH1 supercomplex. Cao P, Bracun L, Yamagata A, Christianson BM, Negami T, Zou B, Terada T, Canniffe DP, Shirouzu M, Li M, Liu LN. Nat Commun 13 1977 (2022)
  15. The role of the γ subunit in the photosystem of the lowest-energy phototrophs. Namoon D, Rudling NM, Canniffe DP. Biochem J 479 2449-2463 (2022)
  16. The structure and assembly of reaction centre-light-harvesting 1 complexes in photosynthetic bacteria. Swainsbury DJK, Qian P, Hitchcock A, Hunter CN. Biosci Rep 43 BSR20220089 (2023)


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