› 7cd7

X-ray diffraction
1.7Å resolution

GFP-40/GFPuv complex, Form I

Released:
DOI: 10.2210/pdb7cd7/pdb
PDB entry 7cd7 coloured by chain, front view.
PDB entry 7cd7 coloured by chain, side view.
PDB entry 7cd7 coloured by chain, top view.
Source organisms:
Primary publication:
A sweet protein monellin as a non-antibody scaffold for synthetic binding proteins.
Yasui N, Nakamura K, Yamashita A
J Biochem 169 585-599 (2021)
PMID: 33386843

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-276210 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Green fluorescent protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 236 amino acids
Theoretical weight: 26.81 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 3-238; Coverage: 99%)
Gene name: GFP
Sequence domains: Green fluorescent protein
GFP-40 Chains: A, C
Molecule details ›
Chains: A, C
Length: 99 amino acids
Theoretical weight: 11.55 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 54.712Å b: 103.168Å c: 67.861Å
α: 90° β: 106.28° γ: 90°
R-values:
R R work R free
0.185 0.182 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Bioaffinity Nanoprobes for Foodborne Pathogen Sensing.
Bruce-Tagoe et al. (2023)
1 more

1 mention without citation

Investigating the Effect of Substituting a Single Cysteine Residue on the Thermal Stability of an Engineered Sweet Protein, Single-Chain Monellin.
Ohnuma et al. (2023)