› 7cel

X-ray diffraction
1.9Å resolution

CBH1 (E217Q) IN COMPLEX WITH CELLOHEXAOSE AND CELLOBIOSE

Released:
DOI: 10.2210/pdb7cel/pdb
PDB entry 7cel coloured by chain, front view.
PDB entry 7cel coloured by chain, side view.
PDB entry 7cel coloured by chain, top view.
Source organism: Trichoderma reesei
Primary publication:
High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei.
Divne C, Ståhlberg J, Teeri TT, Jones TA
J Mol Biol 275 309-25 (1998)
PMID: 9466911

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Exoglucanase 1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 46.07 KDa
Source organism: Trichoderma reesei
Expression system: Trichoderma reesei
UniProt:
  • Canonical: P62694 (Residues: 18-451; Coverage: 88%)
Gene name: cbh1
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand CO 2 x CO
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-D
Spacegroup: I222
Unit cell:
a: 83.7Å b: 84.1Å c: 111.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.215
Expression system: Trichoderma reesei

Quick links

Citations

26 review citations

Hyaluronidases: their genomics, structures, and mechanisms of action.
Stern et al. (2006)
25 more

15 mentions without citation

Thermostable enzymes as biocatalysts in the biofuel industry.
Yeoman et al. (2010)
14 more