› 7cfm

Electron Microscopy
3Å resolution

Cryo-EM structure of the P395-bound GPBAR-Gs complex

Released:
DOI: 10.2210/pdb7cfm/pdb
PDB entry 7cfm coloured by chain, front view.
PDB entry 7cfm coloured by chain, side view.
PDB entry 7cfm coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of GPBAR activation and bile acid recognition.
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature 587 499-504 (2020)
PMID: 32698187
Related structures: EMD-30344

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-276223 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.49 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Nanobody-35 Chain: N
G-protein coupled bile acid receptor 1 Chain: R

Ligands and Environments

2 bound ligands:
Ligand FWX 1 x FWX
Ligand CLR 3 x CLR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3Å
Relevant EMDB volumes: EMD-30344
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

8 review citations

Farnesoid X receptor (FXR): Structures and ligands.
Jiang et al. (2021)
7 more

16 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
15 more