› 7cge

Electron Microscopy
2.9Å resolution

The overall structure of nucleotide free MlaFEDB complex

Released:
DOI: 10.2210/pdb7cge/pdb
PDB entry 7cge coloured by chain, front view.
PDB entry 7cge coloured by chain, side view.
PDB entry 7cge coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural mechanism of phospholipids translocation by MlaFEDB complex.
Chi X, Fan Q, Zhang Y, Liang K, Wan L, Zhou Q, Li Y
Cell Res 30 1127-1135 (2020)
PMID: 32884137
Related structures: EMD-30355

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-159096 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Intermembrane phospholipid transport system permease protein MlaE Chains: A, D
Molecule details ›
Chains: A, D
Length: 260 amino acids
Theoretical weight: 27.89 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P64606 (Residues: 1-260; Coverage: 100%)
Gene names: JW3161, b3194, mlaE, yrbE
Sequence domains: Permease MlaE
Intermembrane phospholipid transport system ATP-binding protein MlaF Chains: B, E
Molecule details ›
Chains: B, E
Length: 269 amino acids
Theoretical weight: 29.13 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P63386 (Residues: 1-269; Coverage: 100%)
Gene names: JW3162, b3195, mlaF, yrbF
Sequence domains: ABC transporter
Intermembrane phospholipid transport system binding protein MlaB Chains: C, F
Molecule details ›
Chains: C, F
Length: 97 amino acids
Theoretical weight: 10.69 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P64602 (Residues: 1-97; Coverage: 100%)
Gene names: JW5535, b3191, mlaB, yrbB
Sequence domains: STAS domain
Intermembrane phospholipid transport system binding protein MlaD Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 183 amino acids
Theoretical weight: 19.59 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P64604 (Residues: 1-183; Coverage: 100%)
Gene names: JW3160, b3193, mlaD, yrbD
Sequence domains: MlaD protein

Ligands and Environments

1 bound ligand:
Ligand PGW 12 x PGW
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-30355
Expression system: Escherichia coli K-12

Quick links

Citations

5 review citations

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
4 more

6 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
5 more