› 7cj8

X-ray diffraction
2.05Å resolution

Crystal structure of N-terminal His-tagged D-allulose 3-epimerase from Methylomonas sp. in complex with D-allulose

Released:
DOI: 10.2210/pdb7cj8/pdb
PDB entry 7cj8 coloured by chain, front view.
PDB entry 7cj8 coloured by chain, side view.
PDB entry 7cj8 coloured by chain, top view.
Source organism: Methylomonas sp. DH-1
Primary publication:
Crystal structure of a novel homodimeric l-ribulose 3-epimerase from Methylomonus sp.
Yoshida H, Yoshihara A, Kato S, Mochizuki S, Akimitsu K, Izumori K, Kamitori S
OpenAccess logo FEBS Open Bio 11 1621-1637 (2021)
PMID: 33838083

Function and Biology Details

Reaction catalysed: 
D-psicose = D-fructose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103101 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-ribulose 3-epimerase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 307 amino acids
Theoretical weight: 33.59 KDa
Source organism: Methylomonas sp. DH-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A172U6X0 (Residues: 1-286; Coverage: 100%)
Gene name: AYM39_05640
Sequence domains: Xylose isomerase-like TIM barrel

Ligands and Environments

3 bound ligands:
Ligand MN 6 x MN
Ligand EPE 6 x EPE
Ligand PSJ 6 x PSJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P1
Unit cell:
a: 51.02Å b: 81.05Å c: 106.13Å
α: 99.3° β: 101.5° γ: 87.24°
R-values:
R R work R free
0.166 0.162 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes.
Xia et al. (2021)

1 mention without citation

Crystal structure of a novel homodimeric l-ribulose 3-epimerase from Methylomonus sp.
Yoshida et al. (2021)